通过电子转移解离质谱法进行肽和蛋白质序列分析。
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry.
作者信息
Syka John E P, Coon Joshua J, Schroeder Melanie J, Shabanowitz Jeffrey, Hunt Donald F
机构信息
Engineering Physics Program, University of Virginia, Charlottesville, VA 22901, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9528-33. doi: 10.1073/pnas.0402700101. Epub 2004 Jun 21.
Abstract
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an electron to multiply protonated peptides in a radio frequency quadrupole linear ion trap (QLT) and induce fragmentation of the peptide backbone along pathways that are analogous to those observed in electron capture dissociation. Modifications to the QLT that enable this ion/ion chemistry are presented, and automated acquisition of high-quality, single-scan electron transfer dissociation MS/MS spectra of phosphopeptides separated by nanoflow HPLC is described.
摘要
本文展示了结合气相离子/离子化学和串联质谱（MS/MS）的肽序列分析方法。单电荷蒽阴离子在射频四极杆线性离子阱（QLT）中将电子转移至多重质子化肽，从而沿与电子捕获解离中观察到的类似途径诱导肽主链断裂。文中介绍了使这种离子/离子化学得以实现的对QLT的改进，并描述了通过纳流高效液相色谱分离的磷酸肽的高质量单扫描电子转移解离MS/MS谱图的自动采集。
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J Am Soc Mass Spectrom. 1994 Nov;5(11):976-89. doi: 10.1016/1044-0305(94)80016-2.
2
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J Proteome Res. 2004 Jan-Feb;3(1):46-54. doi: 10.1021/pr034054u.
3
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