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本文引用的文献

1
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.一种将肽的串联质谱数据与蛋白质数据库中氨基酸序列相关联的方法。
J Am Soc Mass Spectrom. 1994 Nov;5(11):976-89. doi: 10.1016/1044-0305(94)80016-2.
2
Affecting proton mobility in activated peptide and whole protein ions via lysine guanidination.通过赖氨酸胍基化影响活化肽和全蛋白离子中的质子迁移率。
J Proteome Res. 2004 Jan-Feb;3(1):46-54. doi: 10.1021/pr034054u.
3
Whole protein dissociation in a quadrupole ion trap: identification of an a priori unknown modified protein.四极杆离子阱中的全蛋白解离：鉴定一种事先未知的修饰蛋白。
Anal Chem. 2004 Feb 1;76(3):720-7. doi: 10.1021/ac034900k.
4
Phosphorylation site identification via ion trap tandem mass spectrometry of whole protein and peptide ions: bovine alpha-crystallin A chain.通过对完整蛋白质和肽离子进行离子阱串联质谱分析鉴定磷酸化位点：牛α-晶状体蛋白A链
Anal Chem. 2003 Dec 1;75(23):6509-16. doi: 10.1021/ac034410s.
5
Determination of post-translational modifications of proteins by high-sensitivity, high-resolution Fourier transform ion cyclotron resonance mass spectrometry.通过高灵敏度、高分辨率傅里叶变换离子回旋共振质谱法测定蛋白质的翻译后修饰。
J Chromatogr A. 2003 Sep 26;1013(1-2):203-13. doi: 10.1016/s0021-9673(03)01127-0.
6
Applications of electron-ion dissociation reactions for analysis of polycationic chitooligosaccharides in Fourier transform mass spectrometry.电子-离子解离反应在傅里叶变换质谱法中用于分析聚阳离子壳寡糖的应用
Anal Chem. 2003 Nov 1;75(21):5994-6001. doi: 10.1021/ac034477f.
7
Improvement of electron capture efficiency by resonant excitation.通过共振激发提高电子俘获效率。
Rapid Commun Mass Spectrom. 2003;17(19):2208-14. doi: 10.1002/rcm.1163.
8
A new approach for plant proteomics: characterization of chloroplast proteins of Arabidopsis thaliana by top-down mass spectrometry.植物蛋白质组学的一种新方法：通过自上而下的质谱法对拟南芥叶绿体蛋白质进行表征。
Mol Cell Proteomics. 2003 Dec;2(12):1253-60. doi: 10.1074/mcp.M300069-MCP200. Epub 2003 Sep 22.
9
Detection of four oxidation sites in viral prolyl-4-hydroxylase by top-down mass spectrometry.通过自上而下的质谱法检测病毒脯氨酰-4-羟化酶中的四个氧化位点。
Protein Sci. 2003 Oct;12(10):2320-6. doi: 10.1110/ps.03244403.
10
Electron capture dissociation and 13C,15N depletion for deuterium localization in intact proteins after solution-phase exchange.溶液相交换后用于完整蛋白质中氘定位的电子捕获解离和¹³C、¹⁵N缺失
Anal Chem. 2003 Jul 1;75(13):3263-6. doi: 10.1021/ac020690k.

通过电子转移解离质谱法进行肽和蛋白质序列分析。

Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry.

作者信息

Syka John E P, Coon Joshua J, Schroeder Melanie J, Shabanowitz Jeffrey, Hunt Donald F

机构信息

Engineering Physics Program, University of Virginia, Charlottesville, VA 22901, USA.

出版信息

Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9528-33. doi: 10.1073/pnas.0402700101. Epub 2004 Jun 21.

DOI:10.1073/pnas.0402700101
PMID:15210983
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC470779/
Abstract

Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an electron to multiply protonated peptides in a radio frequency quadrupole linear ion trap (QLT) and induce fragmentation of the peptide backbone along pathways that are analogous to those observed in electron capture dissociation. Modifications to the QLT that enable this ion/ion chemistry are presented, and automated acquisition of high-quality, single-scan electron transfer dissociation MS/MS spectra of phosphopeptides separated by nanoflow HPLC is described.

摘要

本文展示了结合气相离子/离子化学和串联质谱（MS/MS）的肽序列分析方法。单电荷蒽阴离子在射频四极杆线性离子阱（QLT）中将电子转移至多重质子化肽，从而沿与电子捕获解离中观察到的类似途径诱导肽主链断裂。文中介绍了使这种离子/离子化学得以实现的对QLT的改进，并描述了通过纳流高效液相色谱分离的磷酸肽的高质量单扫描电子转移解离MS/MS谱图的自动采集。