Wang Xiaoli, Yu Yikai, Li Huawen, Xu Shunqing
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2004 May;33(3):261-3.
To investigate the damages of peroxynitrite (ONOO-) to L-02 and HepG2 cell lines.
Use MTT singe cell gel electrophoresis and flow cytometry was applied to detect the effects of peroxynitrite on cell viability, DNA impairment and cell apoptosis level in two cell lines.
Peroxynitrite had cell toxicity on both cell lines. It caused DNA damages, cell apoptosis and cell cycle change. Furthermore, the damage level was proportional to the concentration of peroxynitrite. The damages were more serious in L-02 in every concentration level than that in HepG2, but no statistic difference between two cell lines.
Peroxynitrite could cause DNA damage, apoptosis and changed cell cycles in both normal and carcinoma cell lines through no statistical significant difference among them was found.
研究过氧亚硝酸盐（ONOO-）对L-02和HepG2细胞系的损伤作用。
采用MTT法、单细胞凝胶电泳法和流式细胞术检测过氧亚硝酸盐对两种细胞系细胞活力、DNA损伤及细胞凋亡水平的影响。
过氧亚硝酸盐对两种细胞系均有细胞毒性，可引起DNA损伤、细胞凋亡及细胞周期改变，且损伤程度与过氧亚硝酸盐浓度呈正比。各浓度水平下，L-02细胞的损伤均比HepG2细胞严重，但两细胞系间差异无统计学意义。
过氧亚硝酸盐可引起正常细胞系和癌细胞系的DNA损伤、凋亡及细胞周期改变，且两者间差异无统计学意义。
