共轭亚油酸对肥胖大鼠PPARγ基因表达及血清瘦素的影响

[Effect of conjugated linoleic acid on PPAR gamma gene expression and serum leptin in obese rat].

作者信息

Zhou Xiaorong, Sun Changhao, Jiang Liying, Wang Haiying

机构信息

School of Public Health, Harbin Medical University, Harbin 150001, China.

出版信息

Wei Sheng Yan Jiu. 2004 May;33(3):307-9.

PMID:15211799

Abstract

OBJECTIVE

In order to study the effect of conjugated linoleic acid on PPAR gamma gene expression, serum leptin, blood glucose and blood lipid level.

METHODS

Five groups of male Wistar rats were randomly selected and fed with different diets (basic diet, high-fat diet, high-fat with 0.75% CLA, high-fat with 1.50% CLA, and high-fat with 3.00% CLA) for 12 weeks. At the end of experiment, the rats were sacrificed, the ratio of lipids to body weight were calculated, and the blood glucose, serum lipids, serum leptin were measured. RT-PCR was used for measuring the expression of PPAR gamma mRNA in white adipose tissue of rats.

RESULTS

It was shown that the supplement of CLA could decrease decreased blood glucose, serum triglyceride, cholesterol and leptin levels, and increased the expression of PPAR gamma in white adipose tissue of obese rat.

CONCLUSION

CLA could decrease blood glucose, serum lipids, and decrease the levels of leptin possibly via activating peroxisome proliferator--activated receptor gamma (PPAR gamma), resulting in the improvement of leptin resistance of obese rat.

摘要

目的

研究共轭亚油酸对PPARγ基因表达、血清瘦素、血糖及血脂水平的影响。

方法

随机选取五组雄性Wistar大鼠，分别给予不同饮食（基础饮食、高脂饮食、含0.75%共轭亚油酸的高脂饮食、含1.50%共轭亚油酸的高脂饮食、含3.00%共轭亚油酸的高脂饮食），喂养12周。实验结束时，处死大鼠，计算脂体比，并检测血糖、血脂、血清瘦素水平。采用RT-PCR法检测大鼠白色脂肪组织中PPARγ mRNA的表达。