Zhou Xiaorong, Sun Changhao, Jiang Liying, Wang Haiying
School of Public Health, Harbin Medical University, Harbin 150001, China.
Wei Sheng Yan Jiu. 2004 May;33(3):307-9.
In order to study the effect of conjugated linoleic acid on PPAR gamma gene expression, serum leptin, blood glucose and blood lipid level.
Five groups of male Wistar rats were randomly selected and fed with different diets (basic diet, high-fat diet, high-fat with 0.75% CLA, high-fat with 1.50% CLA, and high-fat with 3.00% CLA) for 12 weeks. At the end of experiment, the rats were sacrificed, the ratio of lipids to body weight were calculated, and the blood glucose, serum lipids, serum leptin were measured. RT-PCR was used for measuring the expression of PPAR gamma mRNA in white adipose tissue of rats.
It was shown that the supplement of CLA could decrease decreased blood glucose, serum triglyceride, cholesterol and leptin levels, and increased the expression of PPAR gamma in white adipose tissue of obese rat.
CLA could decrease blood glucose, serum lipids, and decrease the levels of leptin possibly via activating peroxisome proliferator--activated receptor gamma (PPAR gamma), resulting in the improvement of leptin resistance of obese rat.
研究共轭亚油酸对PPARγ基因表达、血清瘦素、血糖及血脂水平的影响。
随机选取五组雄性Wistar大鼠，分别给予不同饮食（基础饮食、高脂饮食、含0.75%共轭亚油酸的高脂饮食、含1.50%共轭亚油酸的高脂饮食、含3.00%共轭亚油酸的高脂饮食），喂养12周。实验结束时，处死大鼠，计算脂体比，并检测血糖、血脂、血清瘦素水平。采用RT-PCR法检测大鼠白色脂肪组织中PPARγ mRNA的表达。
结果表明，补充共轭亚油酸可降低肥胖大鼠的血糖、血清甘油三酯、胆固醇和瘦素水平，并增加白色脂肪组织中PPARγ的表达。
共轭亚油酸可能通过激活过氧化物酶体增殖物激活受体γ（PPARγ）降低血糖、血脂水平，降低瘦素水平，从而改善肥胖大鼠的瘦素抵抗。
