Xiang Dayong, Pei Guoxian, Qiu Yurong
Department of Orthopaedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou, Guangdong, 510515, P. R. China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 May;18(3):232-5.
To investigate the value of monitoring cytokines in serum after human double-hand allotransplantation.
We detected solube interleukin-2 receptor(sIL-2R), interleukin-2(IL-2) and interleukin-6(IL-6) levels of the serum in the patient who were performed double-hand transplantation with immunosuppressive from September 2000 to November 2000, and in 15 healthy volunteers by using enzyme linked immunosorbent assays.
The IL-2 and IL-6 levels decreased quickly during inducing period (P < 0.05), and then increased gradually. While the sIL-2R level increased. The IL-2 level was lower(P < 0.05), the IL-6 and sIL-2R levels were slightly higher when compared with those before transplantation. There was significant difference in the IL-2 level between the patient and healthy volunteers both before and after transplantation, in the IL-6 level between the patient and healthy volunteers during inducing period.
IL-2, IL-6 and sIL-2R levels of serum may be useful in diagnosis of rejection, prevention of opportunistic infection, tumor and graft versus host disease.
探讨监测人双手同种异体移植术后血清中细胞因子的价值。
采用酶联免疫吸附测定法，检测2000年9月至2000年11月行双手移植并接受免疫抑制治疗患者血清中的可溶性白细胞介素-2受体（sIL-2R）、白细胞介素-2（IL-2）和白细胞介素-6（IL-6）水平，以及15名健康志愿者血清中的上述细胞因子水平。
诱导期IL-2和IL-6水平迅速下降（P<0.05），随后逐渐升高，而sIL-2R水平升高。与移植前相比，IL-2水平较低（P<0.05），IL-6和sIL-2R水平略高。移植前后患者与健康志愿者之间IL-2水平存在显著差异，诱导期患者与健康志愿者之间IL-6水平存在显著差异。
血清中IL-2、IL-6和sIL-2R水平可能有助于排斥反应的诊断、机会性感染、肿瘤及移植物抗宿主病的预防。
