发育中的卵巢卵泡可抑制假孕大鼠内毒素诱导的肾小球炎症反应。
Developing ovarian follicles inhibit the endotoxin-induced glomerular inflammatory reaction in pseudopregnant rats.
作者信息
Faas Marijke M, van der Schaaf Gerda, Moes Henk, Heineman Maas Jan, de Vos Paul
机构信息
Transplantation Biology and Immunoendocrinology, Division of Medical Biology, Department of Pathology and Laboratory Medicine, University of Groningen, The Netherlands.
出版信息
Am J Reprod Immunol. 2004 May;51(5):385-9. doi: 10.1111/j.1600-0897.2004.00173.x.
PROBLEM
We tested the hypothesis that developing ovarian follicles produce factors inhibiting the endotoxin induced inflammatory response.
METHOD OF STUDY
Pseudopregnant rats were treated with FSH to induced follicular development (FSH-rats). For control we used untreated pseudopregnant rats (PSP-rats) and rats in the follicular phase of the ovarian cycle (C-rats). All rats were infused with either saline or endotoxin. Three days after the infusion rats were sacrificed and kidney specimens were snapfrozen. Cryostat kidney sections were stained for the presence of monocytes, granulocytes, CD11a- and CD11b-positive cells and for ICAM-1 expression.
RESULTS
The results show that induction of follicular development in pseudopregnant rats inhibited glomerular infiltration of monocytes and CD11b(+) cells, while it did not affect the other parameters, i.e. glomerular granulocyte number, CD11a(+) cells and glomerular ICAM-1 expression.
CONCLUSION
Developing ovarian follicles produce factors inhibiting monocyte responses to endotoxin.
问题
我们检验了一个假设，即发育中的卵巢卵泡会产生抑制内毒素诱导的炎症反应的因子。
研究方法
用促卵泡激素（FSH）处理假孕大鼠以诱导卵泡发育（FSH处理组大鼠）。作为对照，我们使用未处理的假孕大鼠（PSP组大鼠）和处于卵巢周期卵泡期的大鼠（C组大鼠）。所有大鼠均输注生理盐水或内毒素。输注后三天处死大鼠，将肾脏标本速冻。对冰冻切片的肾脏进行染色，以检测单核细胞、粒细胞、CD11a和CD11b阳性细胞的存在情况以及细胞间黏附分子-1（ICAM-1）的表达。
结果
结果表明，假孕大鼠卵泡发育的诱导抑制了单核细胞和CD11b（+）细胞的肾小球浸润，而对其他参数没有影响，即肾小球粒细胞数量、CD11a（+）细胞和肾小球ICAM-1表达。
结论
发育中的卵巢卵泡会产生抑制单核细胞对内毒素反应的因子。
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