Hitchcock Robert A, Zeiner Gusti M, Sturm Nancy R, Campbell David A
Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, 4825 Molecular Sciences Building, 609 Charles E. Young Drive, 90095-1489, USA.
FEMS Microbiol Lett. 2004 Jul 1;236(1):73-8. doi: 10.1016/j.femsle.2004.05.020.
Post-transcriptional 3' end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3' ends of mature RNAs is essential for defining 3'-processing activities. We have mapped the mature 3' ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear RNAs, the U3 small nucleolar RNA, and the 5S and 5.8S ribosomal RNAs by ligation-mediated PCR in Trypanosoma brucei. With the exception of U5, two classes of 3' ends were observed: flush with the base of a stem-loop structure, and 1-2 nt extended from a stem-loop. Multiple mechanisms and structural features are likely to influence 3' end maturation of RNAs.
转录后3'末端形成是大多数小RNA成熟过程中的关键步骤。了解成熟RNA精确的3'末端对于定义3'加工活性至关重要。我们通过连接介导的PCR在布氏锥虫中绘制了剪接前导RNA、U1、U2、U4、U5和U6小核RNA、U3小核仁RNA以及5S和5.8S核糖体RNA的成熟3'末端图谱。除U5外，观察到两类3'末端：与茎环结构的基部平齐，以及从茎环延伸1-2个核苷酸。多种机制和结构特征可能影响RNA的3'末端成熟。
