苏云金芽孢杆菌杀蚊幼虫Cry4Aa突变毒素功能形式的结晶及初步晶体学研究

Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin.

作者信息

Boonserm Panadda, Angsuthanasombat Chanan, Lescar Julien

机构信息

Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand.

出版信息

Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1315-8. doi: 10.1107/S0907444904011205. Epub 2004 Jun 22.

DOI:10.1107/S0907444904011205

PMID:15213403

Abstract

The 65 kDa functional form of the mosquito-larvicidal Cry4Aa-R235Q mutant toxin has been crystallized. The crystals belong to space group C222(1), with unit-cell parameters a = 91.2, b = 202.1, c = 98.7 A, and contain one molecule per asymmetric unit. The crystals diffract to approximately 2.9 A using synchrotron radiation and a complete native data set has been collected. The structure has been solved using a molecular-replacement method with the Cry4Ba toxin protein as a search model.

摘要

杀蚊幼虫的Cry4Aa-R235Q突变毒素的65 kDa功能形式已结晶。晶体属于空间群C222(1)，晶胞参数a = 91.2、b = 202.1、c = 98.7 Å，每个不对称单元包含一个分子。使用同步辐射，晶体的衍射分辨率约为2.9 Å，并且已收集完整的天然数据集。该结构已通过分子置换法，以Cry4Ba毒素蛋白作为搜索模型得以解析。