Pellestor Franck, Paulasova Petra
CNRS UPR 1142, Institute of Human Genetics, 141 rue de la Cardonille, F-34396 Montpellier Cedex 5, France.
Eur J Hum Genet. 2004 Sep;12(9):694-700. doi: 10.1038/sj.ejhg.5201226.
Peptide nucleic acids (PNAs) are synthetic mimics of DNA in which the deoxyribose phosphate backbone is replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNAs hybridize with complementary DNAs or RNAs with remarkably high affinity and specificity, essentially because of their uncharged and flexible polyamide backbone. The unique physico-chemical properties of PNAs have led to the development of a variety of research assays, and over the last few years, the use of PNAs has proven their powerful usefulness in molecular biology procedures and diagnostic assays. The more recent applications of PNA involve their use as molecular hybridization probes. Thus, several sensitive and robust PNA-dependent methods have been designed for developing antigene and anticancer drugs, modulating PCR reactions, detecting genomic mutation or labelling chromosomes in situ.
肽核酸（PNA）是DNA的合成模拟物，其中脱氧核糖磷酸骨架被核碱基连接到的假肽聚合物所取代。PNA与互补的DNA或RNA以极高的亲和力和特异性杂交，主要是因为它们不带电荷且具有柔性的聚酰胺骨架。PNA独特的物理化学性质导致了各种研究分析方法的发展，在过去几年中，PNA的应用已证明其在分子生物学程序和诊断分析中非常有用。PNA的最新应用涉及将其用作分子杂交探针。因此，已经设计了几种灵敏且可靠的基于PNA的方法来开发抗基因和抗癌药物、调节PCR反应、检测基因组突变或原位标记染色体。
