通过彗星试验评估3-氨基黄酮的顺铂II配合物与顺铂相比在A549细胞中的遗传毒性。

Genotoxicity of cis-PtII complex of 3-aminoflavone in comparison with cis-DDP in A549 cells evaluated by comet assay.

作者信息

Kosmider Beata, Zyner Elzbieta, Osiecka Regina, Ochocki Justyn

机构信息

Department of Cytogenetics and Plant Molecular Biology, University of Lodz, Poland.

出版信息

Can J Physiol Pharmacol. 2004 May;82(5):353-8. doi: 10.1139/y04-021.

DOI:10.1139/y04-021

PMID:15213736

Abstract

Cis-diamminedichloroplatinum(II) (cis-DDP) is one of the most successful antineoplastic drugs. However, besides effectiveness it gives many side effects. Therefore, current studies are concentrated on searching for new analogs equally effective in chemotherapy but less toxic. Comparison of genotoxic properties of cis-Pt(II) complex of 3-aminoflavone and cis-DDP in a comet assay with and without H2O2 application was performed in A549 cell line. The higher tail moment values were noticed for the former compound contrary to the latter one in both variants. It suggests mainly DNA breaks (besides cross-links) appearance after cis-Pt(II) complex of 3-aminoflavone application and might indicate DNA degradation in comparison with cis-DDP.

摘要

顺二氯二氨铂（II）（顺铂）是最成功的抗肿瘤药物之一。然而，它除了有效之外还会产生许多副作用。因此，目前的研究集中在寻找在化疗中同样有效但毒性较小的新类似物。在A549细胞系中，采用彗星试验比较了3-氨基黄酮的顺铂（II）配合物和顺铂在有无过氧化氢作用下的遗传毒性特性。在两种变体中，前者化合物的尾矩值均高于后者。这主要表明在应用3-氨基黄酮的顺铂（II）配合物后出现了DNA断裂（除交联外），与顺铂相比可能表明存在DNA降解。