Kargol Armin
Loyola University, Department of Physics, 6363 St. Charles Ave., New Orleans, LA 70118, USA.
Cell Mol Biol Lett. 2004;9(2):375-88.
Standard electrophysiology techniques study relaxation transients in voltage-gated ion channels generated by discrete voltage steps. The nonequilibrium response spectroscopy involves analyzing responses to fluctuating potentials. We apply the ensemble NRS method to gating kinetics of Shaker potassium ion channels. We evaluate various proposed Markov models of channel gating from the nonequilibrium response viewpoint. These new NRS protocols can be used to test otherwise indistinguishable models or improve estimates for parameters of channel kinetics models.
标准电生理技术研究由离散电压阶跃产生的电压门控离子通道中的松弛瞬变。非平衡响应光谱法涉及分析对波动电位的响应。我们将系综非平衡响应光谱法应用于Shaker钾离子通道的门控动力学。我们从非平衡响应的角度评估各种提出的通道门控马尔可夫模型。这些新的非平衡响应光谱法协议可用于测试原本难以区分的模型，或改进通道动力学模型参数的估计。
