Guo Zhihui, Shen Yan, Zhao Feng, Wang Mingkui, Dong Shaojun
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Analyst. 2004 Jul;129(7):657-63. doi: 10.1039/b314645f. Epub 2004 Jun 7.
The electrochemical and electrogenerated chemiluminescence of Ru(bpy)(3)(2+) immobilized in clay/Ru(bpy)(3)(2+) multilayer films by layer-by-layer assembly were investigated. The stable multilayer films of clay and Ru(bpy)(3)(2+) were assembled by alternate adsorption of negatively charged clay platelets and positively charged Ru(bpy)(3)(2+) from their aqueous dispersions. UV-vis spectroscopy, quartz crystal microbalance (QCM), cyclic voltammetry, and electrogenerated chemiluminescence (ECL) were used to monitor the immobilization of Ru(bpy)(3)(2+) and the regular growth of the clay/Ru(bpy)(3)(2+) multilayer films. The multilayer films modified electrode was used for the ECL detection of tripropylamine (TPA) and oxalate. The proposed novel immobilized method exhibited good stability, reproducibility and high sensitivity for the determination of TPA and oxalate, which mainly resulted from the contributing of clay nanoparticles with appreciable surface area, special structural features and unusual intercalation properties. Detection limits were 20 and 100 nM for TPA and oxalate, respectively and the linear concentration range extended from 60 nM to 0.66 mM for TPA.
研究了通过层层组装固定在[粘土/Ru(bpy)(3)(2+)]（n）多层膜中的Ru(bpy)(3)(2+)的电化学和电致化学发光。通过从其水分散体中交替吸附带负电的粘土薄片和带正电的Ru(bpy)(3)(2+)，组装了粘土和Ru(bpy)(3)(2+)的稳定多层膜。采用紫外可见光谱、石英晶体微天平（QCM）、循环伏安法和电致化学发光（ECL）来监测Ru(bpy)(3)(2+)的固定化以及[粘土/Ru(bpy)(3)(2+)]（n）多层膜的规则生长。多层膜修饰电极用于三丙胺（TPA）和草酸盐的ECL检测。所提出的新型固定化方法对TPA和草酸盐的测定具有良好的稳定性、重现性和高灵敏度，这主要归因于具有可观表面积、特殊结构特征和异常插层性质的粘土纳米颗粒的作用。TPA和草酸盐的检测限分别为20和100 nM，TPA的线性浓度范围从60 nM扩展到0.66 mM。
