Hosgor Munevver, Karaca Irfan, Ozer Erdener, Erdag Gulsun, Ulukus Cagnur, Fescekoglu Orhan, Aikawa Masanori
Department of Pediatric Surgery, Dr. Behcet Uz Children's Hospital, Izmir, Turkey.
J Pediatr Surg. 2004 Jul;39(7):1018-23. doi: 10.1016/j.jpedsurg.2004.03.056.
BACKGROUND/PURPOSE: Development of indirect inguinal hernia and hydrocele in childhood is readily explained by the persistence of smooth muscle component around the processus vaginalis (PV) after the descent of the testis into the scrotum. The aim of this study was to investigate the expression of smooth muscle myosin heavy chain (SM MHC) isoforms as the markers of smooth muscle cell (SMC) differentiation in childhood inguinal hernia and hydrocele and in age-matched controls.
The authors analyzed sacs from patients with inguinal hernia (male, 10; female, 10) and hydrocele (n = 10) immunohistochemically using monoclonal antibodies against alpha-smooth muscle actin, SM1, SM2 and SMemb. Peritoneal samples (male, 5; female, 5) obtained from age-matched patients served as controls. Immunostaining was evaluated with semiquantitative scoring and chi2 test.
The expression pattern of SM MHC isoforms did not differ among sacs obtained from female inguinal hernia when compared with that of controls. However, strong expression of SMemb within the sac walls of male inguinal hernia and SM1 in hydrocele groups were observed.
Our results indicate that SMC differentiation may play an important role in the obliteration of processus vaginalis in male inguinal hernia and hydrocele after the descent of the testis.
背景/目的：小儿腹股沟斜疝和鞘膜积液的形成，很容易用睾丸降入阴囊后鞘突（PV）周围平滑肌成分持续存在来解释。本研究的目的是调查平滑肌肌球蛋白重链（SM MHC）亚型作为平滑肌细胞（SMC）分化标志物在小儿腹股沟疝和鞘膜积液以及年龄匹配对照组中的表达情况。
作者使用抗α平滑肌肌动蛋白、SM1、SM2和SMemb的单克隆抗体，对腹股沟疝患者（男性10例，女性10例）和鞘膜积液患者（n = 10）的囊进行免疫组织化学分析。从年龄匹配患者获取的腹膜样本（男性5例，女性5例）作为对照。通过半定量评分和卡方检验评估免疫染色情况。
与对照组相比，女性腹股沟疝囊内SM MHC亚型的表达模式无差异。然而，在男性腹股沟疝囊壁内观察到SMemb的强表达，在鞘膜积液组中观察到SM1的强表达。
我们的结果表明，在睾丸下降后，SMC分化可能在男性腹股沟疝和鞘膜积液中鞘突的闭塞过程中起重要作用。
