Chernysheva F A, Alekseeva V Ia, Polygalova O O, Gordon L Kh
Institute of Biochemistry and Biophysics, Research Center RAS, Kazan.
Tsitologiia. 2004;46(3):221-8.
Changes in the localization of ATPase activity, respiration and ultrastructure of wheat root cells with modulated ion conductivity of plasma membrane were studied. A 2 h treatment of excised root with valinomycin (20 microM), N,N-dicyclohexylcarbodiimid (100 microM), gramicidin S (20 microM) and chlorpromazine (100 microM) caused an increased loss of potassium by cells, a decreased respiration and changes in the localization of ATPase activity and in cell ultrastructure. Differences in the observed changes may be conditioned by different mechanisms of action of the membrane active compounds used. It is concluded that changes in the localization of ATPase activity and ultrastructure may indicate some early specific responses of root cells, whereas the increase in the ion conductivity and decrease in respiration under disruption of ion homeostasis caused by membrane active compounds indicate unspecific responses of cells.
研究了质膜离子电导率被调节的小麦根细胞中ATP酶活性、呼吸作用及超微结构定位的变化。用缬氨霉素（20微摩尔）、N,N -二环己基碳二亚胺（100微摩尔）、短杆菌肽S（20微摩尔）和氯丙嗪（100微摩尔）对离体根进行2小时处理，导致细胞钾流失增加、呼吸作用降低以及ATP酶活性定位和细胞超微结构发生变化。观察到的变化差异可能由所用膜活性化合物的不同作用机制所决定。得出的结论是，ATP酶活性定位和超微结构的变化可能表明根细胞的一些早期特异性反应，而由膜活性化合物引起的离子稳态破坏下离子电导率增加和呼吸作用降低则表明细胞的非特异性反应。
