Fèvre-Montange M, Dumontel C, Chevallier P, Isnard A K, Guigard M-P, Trouillas J
INSERM U433, Faculté de Médecine, Lyon-RTH Laennec, Lyon, France.
J Neuroendocrinol. 2004 Jul;16(7):571-6. doi: 10.1111/j.1365-2826.2004.01203.x.
The distribution of transforming growth factor beta (TGFbeta) in the rat and human hypothalamus and neurohypophysis was investigated by immunocytochemical techniques using rabbit polyclonal antisera against TGFbeta(1) and TGFbeta(3). Colocalization of TGFbeta(1) or TGFbeta(3) and arginine vasopressin (AVP) in the rat hypothalamus was studied by double immunolabelling in light microscopy, while their subcellular localization in the rat neurohypophysis was investigated by immunoelectron microscopy. TGFbeta(1) and TGFbeta(3) immunoreactivity was demonstrated in the cell bodies and processes of neurones in the supraoptic nucleus (SON) and paraventricular nucleus (PVN). The TGFbeta-immunoreactive cells were more numerous in the SON compared to the PVN. TGFbeta/AVP double-labelled cells were seen in both nuclei, but some neurones in the SON were labelled for TGFbeta(1) or TGFbeta(3), although not for AVP. In the rat and human neurohypophysis, TGFbeta(3) immunolabelling was more diffuse and stronger than TGFbeta(1) immunolabelling. TGFbeta(1) expression was seen in axonal vesicles and in neurosecretory granules of the axonal endings, while TGFbeta(3) was observed in axonal fibres. Colocalization of TGFbeta(3) or TGFbeta(1) and AVP was observed in some neurosecretory granules, but many were either single-labelled for TGFbeta or AVP or unlabelled. Our results demonstrate, for the first time, the colocalization of TGFbeta and neurohypophysial hormones in magnocellular neurones. We suggest that TGFbeta secreted by the neurohypophysis regulates the proliferation and secretion of certain anterior pituitary cells.
采用抗转化生长因子β（TGFβ）（1）和TGFβ（3）的兔多克隆抗血清，运用免疫细胞化学技术研究了转化生长因子β在大鼠和人类下丘脑及神经垂体中的分布。通过光学显微镜下的双重免疫标记研究了大鼠下丘脑中TGFβ（1）或TGFβ（3）与精氨酸加压素（AVP）的共定位，同时通过免疫电子显微镜研究了它们在大鼠神经垂体中的亚细胞定位。在视上核（SON）和室旁核（PVN）的神经元胞体和突起中显示出TGFβ（1）和TGFβ（3）免疫反应性。与PVN相比，SON中TGFβ免疫反应性细胞更多。在两个核中均可见TGFβ/AVP双标记细胞，但SON中的一些神经元标记了TGFβ（1）或TGFβ（3），但未标记AVP。在大鼠和人类神经垂体中，TGFβ（3）免疫标记比TGFβ（1）免疫标记更弥散且更强。在轴突小泡和轴突末梢的神经分泌颗粒中可见TGFβ（1）表达，而在轴突纤维中观察到TGFβ（3）。在一些神经分泌颗粒中观察到TGFβ（3）或TGFβ（1）与AVP的共定位，但许多颗粒要么仅标记了TGFβ或AVP，要么未标记。我们的结果首次证明了TGFβ与神经垂体激素在大细胞神经元中共定位。我们认为神经垂体分泌的TGFβ调节某些垂体前叶细胞的增殖和分泌。
