Toll样受体在人子宫内膜上皮细胞及细胞系中的表达。
Expression of Toll-like receptors in human endometrial epithelial cells and cell lines.
作者信息
Young Steven L, Lyddon Terri D, Jorgenson Rebecca L, Misfeldt Michael L
机构信息
Department of Obstetrics and Gynecology, School of Medicine, University of Missouri, Columbia, MO, USA.
出版信息
Am J Reprod Immunol. 2004 Jul;52(1):67-73. doi: 10.1111/j.1600-0897.2004.00189.x.
PROBLEM
Are toll-like receptors (TLRs) expressed by human endometrium and endometrial cell lines?
METHODS OF STUDY
Expression of each TLR mRNA species was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of proliferative-phase human endometrium, separated endometrial epithelial cells, and the Ishikawa and RL95-2 endometrial epithelial cell lines. U-937 and SKW 6.4 cell lines were used as positive controls. Functional relevance of PCR findings was tested by enzyme-linked immunsorbent assay (ELISA)analysis of IL-8 production after stimulating cell lines with ligands for TLR2-5.
RESULTS
TLR1-6 and 9 mRNA species were detected in both whole endometrium and separated endometrial epithelial cells. Ishikawa cells expressed TLR2 and TLR5, while RL95-2 cells expressed TLR3, 5, and 9. Response of RL95-2, Ishikawa, and U-937 cells to TLR2-5 ligands was consistent with RT-PCR findings except response to flagellin by Ishikawa cells.
CONCLUSION
These studies provide the first evidence of TLR expression in the endometrium of any species and suggest the usefulness of endometrial cell lines to study TLR function.
问题
人子宫内膜和子宫内膜细胞系是否表达 Toll 样受体（TLR）？
研究方法
通过逆转录聚合酶链反应（RT-PCR）分析增殖期人子宫内膜、分离的子宫内膜上皮细胞以及 Ishikawa 和 RL95-2 子宫内膜上皮细胞系，来确定每种 TLR mRNA 的表达情况。U-937 和 SKW 6.4 细胞系用作阳性对照。在用 TLR2 - 5 的配体刺激细胞系后，通过酶联免疫吸附测定（ELISA）分析白细胞介素 - 8 的产生，来检测 PCR 结果的功能相关性。
结果
在整个子宫内膜和分离的子宫内膜上皮细胞中均检测到 TLR1 - 6 和 9 mRNA。Ishikawa 细胞表达 TLR2 和 TLR5，而 RL95 - 2 细胞表达 TLR3、5 和 9。RL95 - 2、Ishikawa 和 U-937 细胞对 TLR2 - 5 配体的反应与 RT-PCR 结果一致，但 Ishikawa 细胞对鞭毛蛋白的反应除外。
结论
这些研究首次提供了任何物种子宫内膜中 TLR 表达的证据，并表明子宫内膜细胞系在研究 TLR 功能方面的有用性。
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