Baeyens A, Thierens H, Vandenbulcke K, De Ridder L, Vral A
Department of Anatomy, Embryology, Histology and Medical Physics, University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium.
Mutagenesis. 2004 Jul;19(4):285-90. doi: 10.1093/mutage/geh029.
To investigate the chromosomal radiosensitivity of lymphocytes in cancer patients the micronucleus (MN) assay is often used and performed on freshly drawn peripheral blood lymphocytes. The use of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines may have a lot of advantages (e.g. large pool of cells) compared with fresh blood samples. In this study we have investigated whether the response of EBV-transformed lymphoblastoid cell lines to irradiation in the G1/S/G2 phases of the cell cycle is the same as in concordant whole blood cultures where primary lymphocytes were irradiated in the G0 phase of the cell cycle. For this study the MN assay (2 Gy) was performed on EBV-transformed cell lines of breast cancer patients and a group of healthy women. Those breast cancer patients were selected who showed an elevated chromosomal radiosensitivity in fresh blood samples in a previous study. The results demonstrated that the enhanced chromosomal radiosensitivity observed in fresh blood cultures of breast cancer patients is not present in EBV-transformed cell lines derived from the same blood samples. Therefore, care must be taken when EBV cell lines are used to assess chromosomal radiosensitivity in breast cancer patients.
为了研究癌症患者淋巴细胞的染色体放射敏感性，微核（MN）试验常被用于新鲜采集的外周血淋巴细胞。与新鲜血液样本相比，使用爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞系可能有很多优势（如大量的细胞）。在本研究中，我们调查了EBV转化的淋巴母细胞系在细胞周期的G1/S/G2期对辐射的反应是否与在同期全血培养中处于细胞周期G0期的原代淋巴细胞受到辐射时的反应相同。在本研究中，对乳腺癌患者和一组健康女性的EBV转化细胞系进行了MN试验（2 Gy）。那些乳腺癌患者是在之前的一项研究中新鲜血液样本显示染色体放射敏感性升高的患者。结果表明，在乳腺癌患者新鲜血液培养中观察到的增强的染色体放射敏感性在源自相同血液样本的EBV转化细胞系中并不存在。因此，在使用EBV细胞系评估乳腺癌患者的染色体放射敏感性时必须谨慎。
