小干扰RNA选择服务器：一个自动化的小干扰RNA寡核苷酸预测服务器。
siRNA Selection Server: an automated siRNA oligonucleotide prediction server.
作者信息
Yuan Bingbing, Latek Robert, Hossbach Markus, Tuschl Thomas, Lewitter Fran
机构信息
Whitehead Institute for Biomedical Research, Bioinformatics and Research Computing, Nine Cambridge Center, Cambridge, MA 02142, USA.
出版信息
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W130-4. doi: 10.1093/nar/gkh366.
Abstract
The Whitehead siRNA (short interfering RNA) Selection Web Server (http://jura.wi.mit.edu/bioc/siRNA) automates the design of short oligonucleotides that can specifically 'knock down' expression of target genes. These short sequences are about 21 nt in length, and when synthesized as double stranded RNA and introduced into cell culture, can reduce or eliminate the function of the target gene. Depending on the length of a gene, there are potentially numerous combinations of possible 21mers. Some experimental evidence has already shown that not all 21mers in a gene have the same effectiveness at silencing gene function. Our tool incorporates published design rules and presents the scientist with information about uniqueness of the 21mers within the genome, thermodynamic stability of the double stranded RNA duplex, GC content, presence of SNPs and other features that may contribute to the effectiveness of a siRNA.
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