ProSight PTM：一种用于通过自上而下质谱法进行蛋白质鉴定和表征的集成环境。
ProSight PTM: an integrated environment for protein identification and characterization by top-down mass spectrometry.
作者信息
LeDuc Richard D, Taylor Gregory K, Kim Yong-Bin, Januszyk Thomas E, Bynum Lee H, Sola Joseph V, Garavelli John S, Kelleher Neil L
机构信息
W.M. Keck Center for Comparative and Functional Genomics, University of Illinois, Urbana, IL 61801, USA.
出版信息
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W340-5. doi: 10.1093/nar/gkh447.
Abstract
ProSight PTM (https://prosightptm.scs.uiuc.edu/) is a web application for identification and characterization of proteins using mass spectra data from 'top-down' fragmentation of intact protein ions (i.e. without any tryptic digestion). ProSight PTM has many tools and graphical features to facilitate analysis of single proteins, proteins in mixtures and proteins fragmented in parallel. Sequence databases from across the phylogenetic tree are supported, with a new database strategy of 'shotgun annotation' used to assist characterization of wild-type proteins. During a database search, data from divergent sources regarding potential mass differences such as polymorphisms, alternate splicing and post-translational modifications are utilized. The user can optionally control how much of this biological variability should be searched.
摘要
ProSight PTM（https://prosightptm.scs.uiuc.edu/）是一个网络应用程序，用于使用完整蛋白质离子“自上而下”碎片化（即无需任何胰蛋白酶消化）产生的质谱数据来鉴定和表征蛋白质。ProSight PTM具有许多工具和图形功能，便于对单个蛋白质、混合物中的蛋白质以及平行碎片化的蛋白质进行分析。支持来自整个系统发育树的序列数据库，并采用一种新的“鸟枪法注释”数据库策略来辅助野生型蛋白质的表征。在数据库搜索过程中，会利用来自不同来源的有关潜在质量差异的数据，如多态性、可变剪接和翻译后修饰。用户可以选择控制应搜索多少这种生物学变异性。
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