野生大豆和大豆中鲍曼-伯克型蛋白酶抑制剂的多基因家族：存在两个BBI-A基因和假基因。

Multigene family for Bowman-Birk type proteinase inhibitors of wild soja and soybean: the presence of two BBI-A genes and pseudogenes.

作者信息

Deshimaru Masanobu, Yoshimi Shingo, Shioi Seijiro, Terada Shigeyuki

机构信息

Department of Chemistry, Faculty of Science, Fukuoka University, Fukuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2004 Jun;68(6):1279-86. doi: 10.1271/bbb.68.1279.

DOI:10.1271/bbb.68.1279

PMID:15215592

Abstract

Genes for Bowman-Birk type protease inhibitors (BBIs) of wild soja (Glycine soja) and soybean (Glycine max) comprise a multigene family. The organization of the genes for wild soja BBIs (wBBIs) was elucidated by an analysis of their cDNAs and the corresponding genomic sequences, and compared with the counterparts in the soybean. The cDNAs encoding three types of wild soja BBIs (wBBI-A, -C, and -D) were cloned. Two subtypes of cDNAs for wBBI-A, designated wBBI-A1 and -A2, were further identified. Similar subtypes (sBBI-A1 and -A2) were also found in the soybean genome. cDNA sequences for wBBIs were highly homologous to those for the respective soybean homologs. Phylogenetic analysis of these cDNAs demonstrated the evolutional proximity between these two leguminae strains.

摘要

野生大豆（Glycine soja）和栽培大豆（Glycine max）中鲍曼-伯克型蛋白酶抑制剂（BBIs）的基因构成了一个多基因家族。通过对野生大豆BBIs（wBBIs）的cDNA及其相应基因组序列进行分析，阐明了这些基因的结构，并与栽培大豆中的对应基因进行了比较。克隆了编码三种野生大豆BBIs（wBBI-A、-C和-D）的cDNA。进一步鉴定出wBBI-A的两种cDNA亚型，分别命名为wBBI-A1和-A2。在栽培大豆基因组中也发现了类似的亚型（sBBI-A1和-A2）。wBBIs的cDNA序列与各自栽培大豆同源物的cDNA序列高度同源。对这些cDNA进行系统发育分析表明，这两种豆科植物品系在进化上亲缘关系较近。