Takahashi Hideyuki, Kuroki Toshiro, Watanabe Yuko, Dejsirilert Surang, Saengsuk Leelaowadee, Yamai Shiro, Watanabe Haruo
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Microbiol Immunol. 2004;48(6):485-7. doi: 10.1111/j.1348-0421.2004.tb03540.x.
Detection of gamma-glutamyl transpeptidase (GGT; ggt ) activity is one of the useful methods for a specific identification of Neisseria meningitidis. However, we previously happened to isolate a ggt -deficient N. meningitidis strain (NIID113) from a healthy carrier. In this study, in order to re-examine the reliability of the marker, we again investigated the GGT activity of 245 N. meningitidis human isolates and identified two other GGT-defective N. meningitidis isolates besides NIID113. The isolation frequency (1.2%) of ggt mutants among human isolates strongly confirmed the 98.8% reliability of GGT activity as the identification marker for N. meningitidis.
检测γ-谷氨酰转肽酶（GGT；ggt）活性是特异性鉴定脑膜炎奈瑟菌的有用方法之一。然而，我们之前偶然从一名健康携带者中分离出一株ggt缺陷型脑膜炎奈瑟菌菌株（NIID113）。在本研究中，为了重新检验该标志物的可靠性，我们再次调查了245株人源脑膜炎奈瑟菌分离株的GGT活性，并在NIID113之外又鉴定出另外两株GGT缺陷型脑膜炎奈瑟菌分离株。人源分离株中ggt突变体的分离频率（1.2%）有力地证实了GGT活性作为脑膜炎奈瑟菌鉴定标志物的可靠性为98.8%。
