Shiba Hiroshi, Park Jong-In, Suzuki Go, Matsushita Masanori, Nou Ill-Sup, Isogai Akira, Takayama Seiji, Watanabe Masao
Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan.
Genes Genet Syst. 2004 Apr;79(2):87-93. doi: 10.1266/ggs.79.87.
Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S15-SP11b was also co-transcribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.
在芸苔属植物中，自交不亲和性（SI）可区分自身和非自身花粉，它受S位点基因调控。在大多数S单倍型中，一组三个高度多态的基因，即SLG、SRK和SP11，位于S位点区域。在本研究中，我们在甘蓝的自交不亲和S15单倍型中发现了重复的SP11基因，即S15-SP11a、S15-SP11b和S15-SP11b'。RNA凝胶印迹和逆转录聚合酶链反应（RT-PCR）分析表明，在花药组织中特异性检测到两种不同大小的SP11转录本：一个0.65 kb的转录本对应于S15-SP11a（在某些情况下，S15-SP11b的外显子1区域也会一起转录），一个1.4 kb的转录本包含重复的三个基因，即S15-SP11a、S15-SP11b和S15-SP11b'的外显子1区域，这三个基因均与基因间隔区相连。
