Robertson C D, Coombs G H
Department of Zoology, University of Glasgow, Scotland, UK.
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):127-32. doi: 10.1111/j.1574-6968.1992.tb05301.x.
Four distinct bands of cysteine proteinase activity were detected when stationary-phase populations of Leishmania mexicana mexicana were subjected to gelatin-SDS-PAGE. The highest mobility band contained at least three isoforms separable by mono Q anion exchange chromatography. These high mobility activities were distinct from all the major amastigote enzymes. Stationary-phase promastigote populations also contained two acid-activable precursor forms of the promastigote-specific band. It is suggested that these promastigote-specific activities occur in the infective metacyclic stage of the parasite and may have a role in parasite survival upon inoculation into a mammal.
当墨西哥利什曼原虫的稳定期群体进行明胶 - SDS - 聚丙烯酰胺凝胶电泳时，检测到四条不同的半胱氨酸蛋白酶活性带。迁移率最高的条带包含至少三种可通过单Q阴离子交换色谱分离的同工型。这些高迁移率活性与所有主要的无鞭毛体酶不同。稳定期前鞭毛体群体还含有前鞭毛体特异性条带的两种酸激活前体形式。有人认为，这些前鞭毛体特异性活性出现在寄生虫的感染性循环后期阶段，并且可能在接种到哺乳动物体内后对寄生虫的存活起作用。
