Turbek C S, Smith D A, Schardl C L
Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.
FEMS Microbiol Lett. 1992 Jul 1;73(1-2):187-90. doi: 10.1016/0378-1097(92)90606-o.
Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of phaseollidin hydratase for phaseollidin was approximately 7 microM.
菜豆（法国豆）产生的抗菌植物抗毒素中包括异戊烯基化异黄酮基辅酮和菜豆凝集素。豆科病原菌菜豆尖镰孢菌（Fusarium solani f. sp. phaseoli）的培养滤液中存在两种酶活性，即基辅酮水合酶和菜豆凝集素水合酶，它们催化植物抗毒素二甲基烯丙基部分的类似水合反应。在羟基磷灰石色谱法继以制备型非变性凝胶电泳过程中，这两种酶几乎一起被纯化。对从非变性凝胶上连续切下的条带洗脱液进行两种活性的测定。虽然它们在非变性凝胶中没有完全分离，但活性图谱表明这两种活性是不同的。菜豆凝集素水合酶对菜豆凝集素的米氏常数约为7微摩尔。
