Kavalco K F, Pazza R, Bertollo L A C, Moreira-Filho O
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, Brazil.
Cytogenet Genome Res. 2004;106(1):107-10. doi: 10.1159/000078567.
The use of improved cytogenetic techniques such as fluorescent in situ hybridization (FISH) has offered important methodologies for cytotaxonomic and evolutionary studies. In particular, the mapping of 5S rDNA sites has proved to be an excellent marker in the study of different organisms and, more recently, in fish. In the present work, the FISH technique was used to map the 5S rDNA sites in the chromosomes of eight neotropical fish species from the Paraíba do Sul river basin, four of these belonging to the order Characiformes, family Characidae, genus Astyanax (A. scabripinnis, A. parahybae, A. giton and A. intermedius) and four to the order Siluriformes, family Loricariidae (Neoplecostomus microps, Harttia loricariformis, Hypostomus affinis and Upsilodus sp.). Karyotype evolution aspects of the analyzed groups are discussed.
荧光原位杂交（FISH）等改进的细胞遗传学技术的应用为细胞分类学和进化研究提供了重要方法。特别是，5S rDNA位点的定位已被证明是研究不同生物体的优秀标记，最近在鱼类研究中也是如此。在本研究中，采用FISH技术对来自南帕拉伊巴河流域的8种新热带鱼类染色体上的5S rDNA位点进行定位，其中4种属于脂鲤目脂鲤科丽脂鲤属（细鳞丽脂鲤、帕拉伊巴丽脂鲤、吉氏丽脂鲤和中间丽脂鲤），另外4种属于鲇形目骨甲鲶科（小眼新甲鲶、洛氏硬头鲶、近缘下口鲶和未定种Upsilodus sp.）。文中还讨论了所分析类群的核型进化方面。
