Scascitelli M, Gustavino B, Pacchierotti F, Spirito F, Rizzoni M
Department of Biology, University of Rome, Tor Vergata, Rome, Italy.
Cytogenet Genome Res. 2004;105(1):57-64. doi: 10.1159/000078010.
Dual-colour FISH painting with alternative fluorescent chromosome-specific probes allowed us to distinguish chromosomes 1, 4, 6 and 14. The purpose was to check whether nondisjunction rates of specific chromosomes involved in heterozygous Robertsonian fusions are independent of the number of trivalents, or an epistatic effect among Rb chromosomes takes place affecting nondisjunction rates. Probes were used on DAPI-stained metaphases of spermatocytes II of laboratory strains of mice with reconstructed karyotypes heterozygous for one, two, three or four Robertsonian metacentrics in an all-acrocentric background. The existence of such epistatic interactions was not verified.
使用替代荧光染色体特异性探针进行双色荧光原位杂交（FISH）分析，使我们能够区分1号、4号、6号和14号染色体。目的是检查参与杂合罗伯逊融合的特定染色体的不分离率是否独立于三价体的数量，或者是否存在影响不分离率的罗伯逊易位（Rb）染色体间上位效应。在具有全近端着丝粒背景、杂合一条、两条、三条或四条罗伯逊中着丝粒染色体核型的实验室小鼠品系的次级精母细胞的DAPI染色中期上使用探针。未证实存在这种上位相互作用。
