Barbieri Federica, Lorenzi Paola, Ragni Nicola, Schettini Gennaro, Bruzzo Cristina, Pedullà Francesco, Alama Angela
Laboratory of Pharmacology and Neuroscience, National Institute for Cancer Research, Genoa, Italy.
Oncology. 2004;66(4):310-5. doi: 10.1159/000078332.
In order to assess the prognostic role of the cell-cycle regulator cyclin D1 in epithelial ovarian cancer, 70 patients have been studied during an observation period of 8 years.
The cyclin D1 protein content was analyzed by Western blotting, and classed as negative, positive and highly positive by densitometric scanning. The relationship between cyclin D1 expression and clinicopathological variables was determined. Univariate and multivariate survival analyses were also carried out.
Patients with highly positive cyclin D1 tumors had shorter overall survival than patients with positive cyclin D1 (median survival 31 vs. 49 months; p = 0.058). Furthermore, in patients with stage III/IV tumors and residual disease greater than 2 cm, cyclin D1 expression significantly influenced clinical outcome (p = 0.047 and 0.040, respectively). In the Cox's regression model, cyclin D1 expression and residual disease were identified as the most important predictors of survival (p = 0.016 and 0.002, respectively). In patients with high cyclin D1 expression and residual disease after debulking surgery greater than 2 cm, the relative risks of death were to 2.48 and 3.7, respectively, compared to their correspondent counterparts.
The overexpression of cyclin D1 is significantly related to a more aggressive tumor phenotype and poor prognosis in ovarian carcinoma.
为评估细胞周期调节因子细胞周期蛋白D1在上皮性卵巢癌中的预后作用，对70例患者进行了为期8年的观察研究。
采用蛋白质免疫印迹法分析细胞周期蛋白D1的蛋白含量，并通过光密度扫描将其分为阴性、阳性和强阳性。确定细胞周期蛋白D1表达与临床病理变量之间的关系。还进行了单因素和多因素生存分析。
细胞周期蛋白D1强阳性肿瘤患者的总生存期短于细胞周期蛋白D1阳性患者（中位生存期分别为31个月和49个月；p = 0.058）。此外，在Ⅲ/Ⅳ期肿瘤且残留病灶大于2 cm的患者中，细胞周期蛋白D1表达显著影响临床结局（分别为p = 0.047和0.040）。在Cox回归模型中，细胞周期蛋白D1表达和残留病灶被确定为生存的最重要预测因素（分别为p = 0.016和0.002）。在细胞周期蛋白D1高表达且减瘤手术后残留病灶大于2 cm的患者中，与相应的对照患者相比，死亡相对风险分别为2.48和3.7。
细胞周期蛋白D1的过表达与卵巢癌更具侵袭性的肿瘤表型及不良预后显著相关。
