Lee Kyung Hee, Bae Sung Hwa, Lee Jae Lyun, Hyun Myung Soo, Kim Sang Hoon, Song Sun Kyo, Kim Hee Sun
Division of Hemato-Oncology, Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
Oncology. 2004;66(3):210-7. doi: 10.1159/000077997.
The serine protease urokinase-type plasminogen (uPA) and its receptor (uPAR) appear to have a major function in tumor invasion and metastasis. Interleukin-8 (IL-8) acts as an angiogenic factor in solid cancer. The purpose of this study was to determine whether the expression of IL-8 and the uPAR gene correlates with clinicopathological parameters in human gastric carcinoma.
We examined the expression of uPAR mRNA and IL-8 mRNA using Northern blot analysis and RT-PCR in 35 gastric carcinomas and the surrounding normal mucosa. Macroscopic and histopathological tumor findings and survival rates were obtained from the patient records.
uPAR and IL-8 mRNA expression levels were higher in most neoplasms compared to the corresponding normal mucosal tissue. A correlation between uPAR and IL-8 expression in tumors was observed (r = 0.447, p < 0.01). uPAR mRNA expression in the tumors correlated well with lymph node metastasis (p < 0.02), and its stage (p < 0.01). The IL-8 MRNA expression in the tumors correlated with diffuse-type gastric cancer (p < 0.05). The survival rates of patients with tumors displaying high uPAR expression levels were significantly lower (p < 0.04) than those of patients without uPAR expression, but patients with IL-8 expression only showed a tendency to a lower survival rate.
These results suggest that uPAR and IL-8 may be important prognostic factors in human gastric carcinomas.
丝氨酸蛋白酶尿激酶型纤溶酶原（uPA）及其受体（uPAR）似乎在肿瘤侵袭和转移中起主要作用。白细胞介素-8（IL-8）在实体癌中作为一种血管生成因子发挥作用。本研究的目的是确定IL-8和uPAR基因的表达是否与人类胃癌的临床病理参数相关。
我们使用Northern印迹分析和RT-PCR检测了35例胃癌及其周围正常黏膜中uPAR mRNA和IL-8 mRNA的表达。从患者记录中获取肿瘤的宏观和组织病理学发现以及生存率。
与相应的正常黏膜组织相比，大多数肿瘤中uPAR和IL-8 mRNA表达水平更高。观察到肿瘤中uPAR和IL-8表达之间存在相关性（r = 0.447，p < 0.01）。肿瘤中uPAR mRNA表达与淋巴结转移（p < 0.02）及其分期（p < 0.01）密切相关。肿瘤中IL-8 mRNA表达与弥漫型胃癌相关（p < 0.05）。uPAR表达水平高的肿瘤患者的生存率明显低于无uPAR表达的患者（p < 0.04），但仅表达IL-8的患者生存率有降低的趋势。
这些结果表明，uPAR和IL-8可能是人类胃癌重要的预后因素。
