Nishikawa Sumio
Department of Biology, Tsururmi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
Anat Sci Int. 2004 Jun;79(2):87-94. doi: 10.1111/j.1447-073x.2004.00073.x.
Activating transcription factor 2 (ATF2) was localized in the ameloblasts of rat incisors by immunohistochemistry. A specific antibody against phosphorylated ATF2 (p-ATF2), which is an activated form of ATF2, was detected from the proliferation zone to maturation ameloblasts just after the transition. In the secretion zone, a transient increase in p-ATF2 was observed in the late secretion ameloblast nuclei, where a stronger reactivity of p-ATF2 extended from 1 mm apical to the transition to the transition zone, whereas ameloblast nuclei in most of the maturation zone exhibited either weak or no reactivity. A similar pattern was also observed in the case of c-Jun immunohistochemistry, except for in most of the maturation zone, where strong c-Jun reactivity was detected. Thus, ATF-2 and c-Jun are deeply involved in amelogenesis and, in particular, ATF2 is related to the proliferation, differentiation, secretion and transition zones.
通过免疫组织化学方法，发现激活转录因子2（ATF2）定位于大鼠切牙的成釉细胞中。在过渡阶段刚结束后，从增殖区到成熟成釉细胞中检测到一种针对磷酸化ATF2（p-ATF2）的特异性抗体，p-ATF2是ATF2的激活形式。在分泌区，晚期分泌期成釉细胞核中观察到p-ATF2短暂增加，p-ATF2较强的反应性从顶端1毫米处延伸至过渡区，而大多数成熟区的成釉细胞核反应性较弱或无反应。c-Jun免疫组织化学也观察到类似模式，但在大多数成熟区检测到较强的c-Jun反应性除外。因此，ATF-2和c-Jun深度参与釉质形成，特别是ATF2与增殖、分化、分泌和过渡区相关。
