Ranger-Moore James, Bartels Peter H, Bozzo Paul, Einspahr Janine, Liu Yun, Saboda Kathylynn, Alberts David S
Arizona Cancer Center, University of Arizona, 1515 North Campbell Avenue, P.O. Box 245024, Tucson, Arizona 85724-05024, USA.
Anal Quant Cytol Histol. 2004 Jun;26(3):155-65.
To assess the ability of karyometric analysis to demonstrate progression of actinic damage as a function of sun exposure in individuals with actinic keratoses (AKs) and to evaluate the stability of that assessment over a 3-month period.
Biopsies from subjects with AKs were obtained from unexposed skin, sun-exposed skin and AK lesions. Subjects used an SPF 50 sunscreen, and 3 months later additional biopsies were taken from sun-exposed and AK sites. A total of 13,300 nuclei were recorded from 31 subjects.
Measures of nuclear abnormality (NA) and effects of sun damage based on discriminant function (DF) scores were derived. Actinic damage levels varied significantly across biopsy site, demonstrating the method's sensitivity. Accrual of actinic damage was demonstrated in sun-exposed skin and AK lesions when all nuclei were examined over 3 months but only for sun-exposed skin when the worst-damaged nuclei were examined. This suggests a ceiling effect of nuclear damage in the progression to abnormality. Within-subject variability was similar for both NA and DF when all nuclei were considered. Among the worst-damaged nuclei (as defined by high DF), DF showed lower within-case variability than NA, perhaps due to a reduction in nuclear heterogeneity.
Karyometry's ability to detect subtle levels of actinic damage in nuclear chromatin patterns may make it useful in screening agents for possible use in cancer chemoprevention.
评估核测量分析在光化性角化病（AK）患者中显示光化性损伤进展与日晒关系的能力，并评估该评估在3个月期间的稳定性。
从未暴露皮肤、日晒皮肤和AK病变处获取AK患者的活检样本。受试者使用防晒系数为50的防晒霜，3个月后从日晒部位和AK部位取额外的活检样本。共记录了31名受试者的13300个细胞核。
得出基于判别函数（DF）评分的核异常（NA）测量值和阳光损伤效应。光化性损伤水平在不同活检部位有显著差异，表明该方法具有敏感性。当在3个月内检查所有细胞核时，日晒皮肤和AK病变处均显示出光化性损伤的累积，但仅检查损伤最严重的细胞核时，仅日晒皮肤有此现象。这表明核损伤在进展为异常过程中存在上限效应。当考虑所有细胞核时，NA和DF的受试者内变异性相似。在损伤最严重的细胞核（由高DF定义）中，DF的病例内变异性低于NA，这可能是由于核异质性降低所致。
核测量分析检测核染色质模式中细微光化性损伤水平的能力，可能使其在筛选可能用于癌症化学预防的药物方面有用。
