Ranger-Moore James, Bozzo Paul, Alberts David, Einspahr Janine, Liu Yun, Thompson Deborah, Stratton Steven, Stratton M Suzanne, Bartels Peter
College of Public Health, Arizona Cancer Center, University of Arizona, Tucson, AZ 85721, USA.
Anal Quant Cytol Histol. 2003 Dec;25(6):353-61.
To use karyometric analysis methods to compare actinic keratoses (AKs) to squamous cell carcinomas (SCCs) to determine if SCCs showed a logical progression beyond that seen in AKs and to explore variability within and between lesion types to better understand distinctions between the 2.
Biopsies from 31 subjects with AKs were obtained from upper inner arm skin, forearm skin and AK lesions. Biopsies from 23 different subjects in a related subproject provided SCC biopsies for comparison.
Karyometric measures of nuclear abnormality and sun damage were derived. Mean actinic damage levels progressed logically from inner arm to sun-exposed skin, to AK, to SCC. Considerable heterogeneity existed at the case level. Unsupervised learning methods revealed 2 distinct clusters of progressed lesions with different nuclear signatures, reflecting differing levels of actinic damage. Number of AKs and SCCs and invasiveness and differentiation of SCCs were distributed across both clusters in roughly equivalent proportions.
Karyometric methods, shown previously to be capable of sensitively detecting subtle nuclear changes, revealed the possibility of 2 progression pathways, each containing AKs and SCCs. This finding may have prognostic implications.
运用核测量分析方法对比光化性角化病（AK）与鳞状细胞癌（SCC），以确定SCC是否呈现出超越AK的合理进展，并探究病变类型内部及之间的变异性，从而更好地理解两者之间的区别。
从31名患有AK的受试者的上臂内侧皮肤、前臂皮肤及AK病变处获取活检样本。在一个相关子项目中，从23名不同受试者处获取SCC活检样本用于比较。
得出了核异常及阳光损伤的核测量指标。平均光化损伤水平从手臂内侧到暴露于阳光下的皮肤，再到AK，最后到SCC，呈合理进展。在病例层面存在相当大的异质性。无监督学习方法揭示了具有不同核特征的2个不同的进展性病变簇，反映了不同程度的光化损伤。AK和SCC的数量以及SCC的侵袭性和分化在两个簇中的分布大致相当。
核测量方法先前已被证明能够灵敏地检测到细微的核变化，该方法揭示了2种进展途径的可能性，每种途径都包含AK和SCC。这一发现可能具有预后意义。
