Lee J H, Rowley W A, Platt K B
Department of Entomology, Iowa State University, Ames 50011-3222, USA.
J Med Entomol. 2000 Jan;37(1):194-7. doi: 10.1603/0022-2585-37.1.194.
Experiments were designed to evaluate the vector competence of Culex tarsalis Coquillet from an area (Sioux City) where Cx. tarsalis is most abundant in Iowa for western equine encephalomyelitis virus (WEE-7738). WEE-7738 was isolated from Aedes trivittatus (Coquillet) collected in Ames, IA, in 1977. Infection rate, dissemination rate, multiplication efficiency, and transmission rate were determined for this virus in the SC strain of Cx. tarsalis. SC strain of Cx. tarsalis was as susceptible to WEE-7738 as Californian strains of Cx. tarsalis were to BFS1703 strain of WEE; OID50 of SC Cx. tarsalis was 2.63 log TCID50 per mosquito and OID50 of Californian strains of Cx. tarsalis were 2.0-4.1 log PFU per mosquito. However, transmission of WEE-7738 (4.2%) by the SC strain of Cx. tarsalis was lower than those (10-60%) reported in other studies.
实验旨在评估来自爱荷华州库蚊数量最多的地区（苏城）的致倦库蚊对西部马脑炎病毒（WEE - 7738）的媒介能力。WEE - 7738于1977年从爱荷华州艾姆斯采集的三带喙库蚊中分离得到。测定了该病毒在致倦库蚊SC品系中的感染率、传播率、增殖效率和传播速率。致倦库蚊SC品系对WEE - 7738的易感性与致倦库蚊加利福尼亚品系对WEE的BFS1703株的易感性相同；致倦库蚊SC品系的半数感染剂量（OID50）为每只蚊子2.63 log TCID50，致倦库蚊加利福尼亚品系的OID50为每只蚊子2.0 - 4.1 log PFU。然而，致倦库蚊SC品系对WEE - 7738的传播率（4.2%）低于其他研究报道的传播率（10 - 60%）。
