Kimura Takafumi, Wang Jianfeng, Matsui Kazuo, Imai Shiro, Yokoyama Shigeki, Nishikawa Mitsuo, Ikehara Susumu, Sonoda Yoshiaki
Department of Molecular-Targeting Cancer Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Int J Hematol. 2004 May;79(4):328-33. doi: 10.1532/ijh97.03158.
Using the intra-bone marrow injection (IBMI) technique, we recently identified human cord blood-derived CD34- severe combined immunodeficiency (SCID)-repopulating cells (SRCs) with extensive lymphomyeloid reconstituting ability. In this study, we further investigated the hematopoietic stem cell (HSC) characteristics of these cells in terms of proliferative and migratory potentials. The absolute numbers of CD45+ and CD34+ cells generated by 1 CD34- SRC are significantly higher than those generated by 1 CD34+ SRC. It is interesting that CD34- SRCs have significantly higher migratory and proliferative abilities than CD34+ SRCs. Moreover, only 2 CD34- SRCs transplanted to primary recipients consistently showed secondary reconstituting capacity. This finding suggested the more homogenous nature of CD34- SRCs than that of the population of CD34+ SRCs. These results provided further evidence that CD34- SRCs are functionally different from CD34+ SRCs and that they are a distinct class of primitive HSCs.
利用骨髓内注射（IBMI）技术，我们最近鉴定出了具有广泛淋巴细胞和髓细胞重建能力的人脐带血来源的CD34-重症联合免疫缺陷（SCID）重建细胞（SRC）。在本研究中，我们进一步从增殖和迁移潜能方面研究了这些细胞的造血干细胞（HSC）特性。1个CD34-SRC产生的CD45+和CD34+细胞的绝对数量显著高于1个CD34+SRC产生的细胞数量。有趣的是，CD34-SRC的迁移和增殖能力明显高于CD34+SRC。此外，仅2个移植到初级受体的CD34-SRC持续显示出二次重建能力。这一发现表明CD34-SRC比CD34+SRC群体具有更均一的性质。这些结果进一步证明CD34-SRC在功能上不同于CD34+SRC，并且它们是一类独特的原始造血干细胞。
