Hajdusek Ondrej, Ditrich Oleg, Slapeta Jan
Institute of Parasitology, Academy of Sciences of the Czech Republic, Ceské Budĕjovice.
Vet Parasitol. 2004 Jul 14;122(3):183-92. doi: 10.1016/j.vetpar.2004.04.005.
To study the diversity of Cryptosporidium spp. in various hosts, we used the variability of the small-subunit rRNA gene and the Cryptosporidium oocyst wall protein genes. Oocysts from humans, cattle, horses, dogs, field mice, chickens, reptiles, deer, goat, cat, antelope and from a sample of water reservoir were assayed. The zoonotic C. parvum bovine genotype sequence was found to be present in the most of isolates. This study shows a complex epidemiology pattern for C. parvum bovine genotype infections. The identification of cattle, horse, and deer isolates emphasizes a transmission route for C. parvum via these hosts, and identifies a potential source for human infection in the Czech Republic. Furthermore, C. andersoni from a cow, C. baileyi from a chicken, C. felis from a cat, C. meleagridis from a dog, and C. saurophilum and C. serpentis from reptiles were also identified in the isolates from the Czech Republic.
为研究隐孢子虫在不同宿主中的多样性，我们利用小亚基核糖体RNA基因和隐孢子虫卵囊壁蛋白基因的变异性。对来自人类、牛、马、狗、田鼠、鸡、爬行动物、鹿、山羊、猫、羚羊以及一个水库水样中的卵囊进行了检测。发现大多数分离株中存在人兽共患的微小隐孢子虫牛基因型序列。这项研究显示了微小隐孢子虫牛基因型感染的复杂流行病学模式。牛、马和鹿分离株的鉴定强调了微小隐孢子虫通过这些宿主的传播途径，并确定了捷克共和国人类感染的潜在来源。此外，在捷克共和国的分离株中还鉴定出了来自牛的安氏隐孢子虫、来自鸡的贝氏隐孢子虫、来自猫的猫隐孢子虫、来自狗的火鸡隐孢子虫以及来自爬行动物的嗜蜥隐孢子虫和蛇隐孢子虫。
