Zhang Z M, Ballinger J R, Sheldon K, Boxen I
Department of Nuclear Medicine, Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Canada.
Int J Rad Appl Instrum B. 1992 Aug;19(6):607-9. doi: 10.1016/0883-2897(92)90094-f.
Monoclonal antibodies can be labelled with technetium-99m by prereduction of the antibody with 2-mercaptoethanol, then reduction of pertechnetate with an aliquot of a stannous kit, resulting in greater than 97% labelling without the need for further purification. The present work shows that equally high labelling can be obtained with a variety of weak ligands and that the optimum quantity of stannous chloride is 2-4 micrograms. Although the label was stable to challenge with excess DTPA, cysteine was able to remove a portion of the label. We have also shown that this technique works with the IgG2a isotype in addition to the previously reported IgG1 isotype. This approach is simple, convenient and reproducible, and warrants further clinical evaluation.
单克隆抗体可通过先用2-巯基乙醇对抗体进行预还原，然后用等量的氯化亚锡试剂盒还原高锝酸盐，用锝-99m进行标记，无需进一步纯化即可实现大于97%的标记率。目前的研究表明，使用各种弱配体也能获得同样高的标记率，且氯化亚锡的最佳用量为2-4微克。尽管标记物对过量二乙三胺五乙酸的挑战具有稳定性，但半胱氨酸能够去除部分标记物。我们还表明，除了先前报道的IgG1同种型外，该技术也适用于IgG2a同种型。这种方法简单、方便且可重复，值得进一步进行临床评估。
