Cheng Liang, MacLennan Gregory T, Zhang Shaobo, Wang Mingsheng, Pan Chong-Xian, Koch Michael O
Department of Pathology and Laboratory Medicine, School of Medicine, Indiana University Medical Center, University Hospital 3465, 550 North University Boulevard, Indianapolis, IN 46202, USA.
Cancer. 2004 Jul 1;101(1):183-8. doi: 10.1002/cncr.20343.
In the 1999 World Health Organization classification system, papillary tumors of the urinary bladder were classified as papilloma, papillary urothelial neoplasm of low malignant potential (PUNLMP), and as Grade 1, Grade 2, and Grade 3 urothelial carcinoma. The biologic potential of PUNLMP of the urinary bladder is controversial. To the authors' knowledge, information regarding the genetic changes of PUNLMP tumors of the bladder is limited.
The authors examined loss of heterogygosity (LOH) at 5 polymorphic microsatellite markers on chromosome 9q32-33 (D9S177), 9p22 (IFNA), 17p13.1 (TP53), 12q14-24 (D12S1051), and 3p25-26 (D3S3050) from 26 patients who were diagnosed with PUNLMP tumors of the urinary bladder. Tumors were microdissected from sections prepared from formalin-fixed, paraffin-processed tissue specimens using laser capture microdissection.
LOH was found in 21 of 26 (81%) patients with PUNLMP. The rate of LOH was 41% with D9S177, 32% with IFNA, 29% with TP53, 26% with D12S1051, and 44% with D3S3050. Allelic loss of multiple chromosome loci was often present in patients with PUNLMP tumors.
Genetic changes that commonly occur in advanced bladder carcinoma (> or = pT2) are frequently found in PUNLMP of the urinary bladder.
在1999年世界卫生组织分类系统中，膀胱乳头状肿瘤被分类为乳头状瘤、低恶性潜能乳头状尿路上皮肿瘤（PUNLMP）以及1级、2级和3级尿路上皮癌。膀胱PUNLMP的生物学潜能存在争议。据作者所知，关于膀胱PUNLMP肿瘤基因改变的信息有限。
作者检测了26例被诊断为膀胱PUNLMP肿瘤患者在9q32 - 33（D9S177）、9p22（IFNA）、17p13.1（TP53）、12q14 - 24（D12S1051）和3p25 - 26（D3S3050）这5个多态性微卫星标记处的杂合性缺失（LOH）。使用激光捕获显微切割技术从福尔马林固定、石蜡处理的组织标本制备的切片中显微切割肿瘤。
26例PUNLMP患者中有21例（81%）发现LOH。D9S177处的LOH率为41%，IFNA处为32%，TP53处为29%，D12S1051处为26%，D3S3050处为44%。PUNLMP肿瘤患者中常出现多个染色体位点的等位基因缺失。
在晚期膀胱癌（≥pT2）中常见的基因改变在膀胱PUNLMP中也经常被发现。
