基因组学和蛋白质组学时代的激光捕获显微切割技术：探寻癌症的遗传基础
Laser capture microdissection in the genomic and proteomic era: targeting the genetic basis of cancer.
作者信息
Domazet Barbara, Maclennan Gregory T, Lopez-Beltran Antonio, Montironi Rodolfo, Cheng Liang
机构信息
Departments of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
出版信息
Int J Clin Exp Pathol. 2008 Mar 15;1(6):475-88.
Abstract
The advent of new technologies has enabled deeper insight into processes at subcellular levels, which will ultimately improve diagnostic procedures and patient outcome. Thanks to cell enrichment methods, it is now possible to study cells in their native environment. This has greatly contributed to a rapid growth in several areas, such as gene expression analysis, proteomics, and metabolonomics. Laser capture microdissection (LCM) as a method of procuring subpopulations of cells under direct visual inspection is playing an important role in these areas. This review provides an overview of existing LCM technology and its downstream applications in genomics, proteomics, diagnostics and therapy.
摘要
新技术的出现使人们能够更深入地洞察亚细胞水平的过程，这最终将改善诊断程序和患者预后。得益于细胞富集方法，现在有可能在细胞的天然环境中对其进行研究。这极大地推动了基因表达分析、蛋白质组学和代谢组学等多个领域的快速发展。激光捕获显微切割（LCM）作为一种在直接目视检查下获取细胞亚群的方法，在这些领域发挥着重要作用。本文综述了现有的LCM技术及其在基因组学、蛋白质组学、诊断和治疗方面的下游应用。
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