胃癌中18号染色体杂合性缺失的等位基因分型。
Allelotyping for loss of heterozygosity on chromosome 18 in gastric cancer.
作者信息
Yu Jing-Cui, Sun Kai-Lai, Liu Buo, Fu Song-Bin
机构信息
Department of Medical Genetics, China Medical University, Shenyang 110001, China.
出版信息
World J Gastroenterol. 2004 Jul 1;10(13):1964-6. doi: 10.3748/wjg.v10.i13.1964.
AIM
To investigate the association between loss of heterozygosity (LOH) on chromosome 18 and sporadic gastric cancer.
METHODS
Multiplex PCR was used to screen 14 highly polymorphic microsatellite markers on chromosome 18 in 45 cases of primary gastric cancer. PCR products were separated on polyacrylamide gels and the electrophoresis maps were analyzed with Genescan and Genotyper.
RESULTS
The LOH frequencies in gastric cancer at all 14 markers ranged from 10% to 58%. Eleven markers were found with over 20% LOH frequencies, in which 9 markers located in 18q, and 2 markers in 18p. Two overlapping deleted regions were identified: R1 between D18S61-D18S1161 at 18q22 (9cM) with 24% LOH frequency; R2 between D18S462-D18S70 at 18q22-23(6cM) with 32% LOH frequency.
CONCLUSION
LOH of chromosome 18 (18q and 18p) may be involved in gastric tumorigenesis. Two overlapping deleted fragments suggested that there might be unidentified tumor suppressor genes in those two regions.
目的
研究18号染色体杂合性缺失（LOH）与散发性胃癌之间的关联。
方法
采用多重聚合酶链反应（Multiplex PCR）对45例原发性胃癌患者18号染色体上的14个高度多态性微卫星标记进行筛选。聚合酶链反应（PCR）产物在聚丙烯酰胺凝胶上分离，并用基因扫描（Genescan）和基因分型（Genotyper）分析电泳图谱。
结果
14个标记在胃癌中的杂合性缺失（LOH）频率在10%至58%之间。发现11个标记的杂合性缺失（LOH）频率超过20%，其中9个标记位于18q，2个标记位于18p。确定了两个重叠缺失区域：R1位于18q22（9cM）的D18S61 - D18S1161之间，杂合性缺失（LOH）频率为24%；R2位于18q22 - 23（6cM）的D18S462 - D18S70之间，杂合性缺失（LOH）频率为32%。
结论
18号染色体（18q和18p）的杂合性缺失（LOH）可能参与胃癌的发生。两个重叠缺失片段提示这两个区域可能存在未被识别的抑癌基因。
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