肺移植受者上皮衬液中巨细胞病毒DNA浓度与症状性巨细胞病毒感染的相关性

Association of cytomegalovirus DNA concentration in epithelial lining fluid and symptomatic cytomegalovirus infection in lung transplant recipients.

作者信息

Zedtwitz-Liebenstein Konstantin, Jaksch Peter, Bauer Claudia, Popow Therese, Klepetko Walter, Hofmann Hanns, Puchhammer-Stöckl Elisabeth

机构信息

Institute of Virology, University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria.

出版信息

Transplantation. 2004 Jun 27;77(12):1897-9. doi: 10.1097/01.tp.0000131154.79019.41.

DOI:10.1097/01.tp.0000131154.79019.41

PMID:15223910

Abstract

BACKGROUND

The authors have investigated the effectiveness of virus detection from bronchoalveolar lavage (BAL) samples for the identification of symptomatic cytomegalovirus (CMV) infection in lung transplant recipients.

METHODS

Thus, 275 BAL samples taken from 105 lung transplant recipients during follow-up were analyzed by quantitative polymerase chain reaction (PCR) and virus isolation.

RESULTS

Quantitative PCR detected virus in all 24 BAL samples taken at onset of symptomatic disease, and virus culture in only 7 samples (29.2%). Twenty-three of 251 BAL samples (9.2%) were positive by PCR, although they were obtained in an asymptomatic phase. Quantitation of CMV DNA from BAL allowed no discrimination between symptomatic and asymptomatic infection in individual cases. However, when the urea dilution method was used to recalculate the CMV DNA concentration for the epithelial lining fluid (ELF) diluted in the BAL, a CMV DNA level of more than 10 copies/mL ELF was clearly associated with symptomatic disease.

CONCLUSION

The CMV DNA level in ELF may be a marker for symptomatic CMV infection.

摘要

背景

作者研究了从支气管肺泡灌洗（BAL）样本中检测病毒对于识别肺移植受者有症状的巨细胞病毒（CMV）感染的有效性。

方法

因此，对105例肺移植受者随访期间采集的275份BAL样本进行了定量聚合酶链反应（PCR）和病毒分离分析。

结果

定量PCR在所有24份有症状疾病发作时采集的BAL样本中均检测到病毒，而病毒培养仅在7份样本中检测到（29.2%）。251份BAL样本中有23份（9.2%）PCR呈阳性，尽管这些样本是在无症状期采集的。对BAL中的CMV DNA进行定量分析无法区分个体病例中的有症状感染和无症状感染。然而，当使用尿素稀释法重新计算BAL中稀释的上皮衬液（ELF）的CMV DNA浓度时，ELF中CMV DNA水平超过10拷贝/mL与有症状疾病明显相关。