He De-Fu, Chen Fu-Jun, Zhou Shao-Ci
Department of Environmental Science and Brain Science Research Center, East China Normal University, Shanghai 200062, China.
Sheng Li Xue Bao. 2004 Jun 25;56(3):374-8.
Experiments were performed on Sprague Dawley rats with multibarrel microelectrode technique. The effects of acoustic response of A I cortex neurons produced by electrical stimulation of lateral amygdaloid nucleus (LA) and the influence of GABA were observed. Experimental results showed that iontophoretic administration of GABA caused a pronounced inhibition of the electrical activity of A-I neurons. Blockade of GABA(A) with bicuculline (BIC) facilitated the acoustic response. The acoustic response of A-I neurons was inhibited when the LA was stimulated. Iontophoretic application of GABA resulted in a similar inhibitory effect as that of LA stimulation. Blockade of GABA(A) with bicuculline reversed the inhibitory effect of LA stimulation on the acoustic response of A-I neurons. In contrast, application of strychnine, a glycine receptor antagonist, could not reverse the inhibitory effect of LA. Baclofen, a GABA(B) agonist, did not affect the acoustic response of the auditory neurons. These results indicate that GABA is the ultimate transmitter which mediates the LA stimulation-induced inhibition of the acoustic response of A-I neurons in rats, possibly via the GABA(A) receptor.
采用多管微电极技术对斯普拉格-道利大鼠进行实验。观察了电刺激杏仁核外侧核（LA）对初级听觉皮层（AI）神经元听觉反应的影响以及γ-氨基丁酸（GABA）的作用。实验结果表明，离子导入GABA可显著抑制AI神经元的电活动。用荷包牡丹碱（BIC）阻断GABA（A）可促进听觉反应。刺激LA时，AI神经元的听觉反应受到抑制。离子导入GABA产生的抑制作用与刺激LA相似。用荷包牡丹碱阻断GABA（A）可逆转LA刺激对AI神经元听觉反应的抑制作用。相反，应用甘氨酸受体拮抗剂士的宁不能逆转LA的抑制作用。GABA（B）激动剂巴氯芬不影响听觉神经元的听觉反应。这些结果表明，GABA是介导LA刺激诱导的大鼠AI神经元听觉反应抑制的最终递质，可能是通过GABA（A）受体发挥作用。
