白细胞介素-10启动子多态性与自身免疫性肝病关系的研究

[A study on the relationship between interleukin-10 promoter polymorphism and autoimmune liver disease].

作者信息

Chen Qu-bo, Fan Lie-ying, Zhong Ren-qian, Tu Xiao-qing, Yuan Yuan, Zhu Ye, Ye Wei-min, Lu Hui-qi, Han Hui-xing

机构信息

The Second Institute of Clinical Medicine, Guangzhou University of TCM, Guangzhou 510120, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2004 Jun;12(6):356-8.

PMID:15225432

Abstract

OBJECTIVE

To investigate whether three biallelic polymorphisms at the position -592, -819 and -1082 in the promoter region of the IL-10 gene were associated with the incidence of autoimmune liver disease.

METHODS

The IL-10 -592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphisms analysis (PCR-RFLP), while polymerase chain reaction- sequence specific primer (PCR-SSP) assay was used to detect IL-10 -819 polymorphisms.

RESULTS

Among 54 Chinese patients with AIH or 77 Chinese patients with PBC versus healthy controls, the frequency of AA, GA genotypes at IL-10 gene promoter -1082 position was 87.0% or 83.1% versus 90.0%, 13.0% or 16.9% versus 10.0%, respectively (P > 0.05), the GG genotype in Chinese populations is absent; the frequency of CC, CT, TT genotypes at IL-10 gene promoter -819 position was 11.11% or 9.1% versus 8.1%, 44.4% or 53.3% versus 45.0%, 44.4% or 37.7% versus 46.9%, respectively (P > 0.05); the frequency of CC, CA, AA genotypes at IL-10 gene promoter -592 position was 4.9% or 14.3% versus 10.0%, 51.2% or 53.3% versus 51.9%, 43.9% or 32.5% versus 38.1%, respectively (P > 0.05). No alleles differed significantly in each groups.

CONCLUSION

There were no association between IL-10 gene polymorphisms and autoimmune liver disease

摘要

目的

研究白细胞介素10（IL-10）基因启动子区域-592、-819和-1082位点的三个双等位基因多态性与自身免疫性肝病的发病率是否相关。

方法

采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）对IL-10 -592和IL-10-1082多态性进行基因分型，同时采用聚合酶链反应-序列特异性引物（PCR-SSP）检测IL-10 -819多态性。

结果

在54例中国自身免疫性肝炎（AIH）患者或77例中国原发性胆汁性胆管炎（PBC）患者与健康对照中，IL-10基因启动子-1082位点AA、GA基因型频率分别为87.0%或83.1%，而健康对照为90.0%、13.0%或16.9%，而健康对照为10.0%（P>0.05），中国人群中不存在GG基因型；IL-10基因启动子-819位点CC、CT、TT基因型频率分别为11.11%或9.1%，而健康对照为8.1%、44.4%或53.3%，而健康对照为45.0%、44.4%或37.7%，而健康对照为46.9%（P>0.05）；IL-10基因启动子-592位点CC、CA、AA基因型频率分别为4.9%或14.3%，而健康对照为10.0%、51.2%或53.3%，而健康对照为51.9%、43.9%或32.5%，而健康对照为38.1%（P>0.05）。各组等位基因频率无显著差异。