Murashov Alexander K, Pak Elena S, Hendricks Wesley A, Tatko Lisa M
Department of Physiology, East Carolina University School of Medicine, Brody Bldg. #6N-98, 600 Moye Blvd., Greenville, NC 27858, USA.
FEBS Lett. 2004 Jul 2;569(1-3):165-8. doi: 10.1016/j.febslet.2004.05.042.
Existing protocols show a variety in the percentage of neurons that can be generated from mouse embryonic stem (ES) cells. In the current study, we compared effects of various differentiating conditions, including gelatin and poly-l-ornithine/fibronectin coatings, and NGF and 17beta-estradiol treatments on the total yield of neurons, as well as, neurite growth and branching. Here, we show that combination of fibronectin coating with 17beta-estradiol increased number of generated neurons over 50%. Poly-l-ornithine/fibronectin increased the percent of neurons in all cultures, suggesting its direct influence on neurogenesis. Addition of 17beta-estradiol reduced mean neurite length in culture, but significantly increased branching. Our results indicate a substrate-dependent regulation of estrogen-induced ES cells differentiation into neuronal cells.
现有方案显示，从小鼠胚胎干细胞（ES细胞）中生成的神经元百分比存在差异。在本研究中，我们比较了各种分化条件的影响，包括明胶和聚-L-鸟氨酸/纤连蛋白包被，以及NGF和17β-雌二醇处理对神经元总产量、神经突生长和分支的影响。在此，我们表明纤连蛋白包被与17β-雌二醇的组合使生成的神经元数量增加了50%以上。聚-L-鸟氨酸/纤连蛋白增加了所有培养物中神经元的百分比，表明其对神经发生有直接影响。添加17β-雌二醇可缩短培养物中神经突的平均长度，但显著增加分支。我们的结果表明雌激素诱导的ES细胞向神经元细胞分化存在底物依赖性调节。
