Kunakornsawat S, Rosol T J, Capen C C, Omdahl J L, Leroy B E, Inpanbutr N
Department of Veterinary Biosciences, The Ohio State University, 1900 Coffey Road, Columbus, OH 43210, USA.
J Steroid Biochem Mol Biol. 2004 May;89-90(1-5):409-12. doi: 10.1016/j.jsbmb.2004.03.009.
The aim of this study was to investigate effects of 1,25(OH)(2)D(3) (calcitriol), 25OHD(3), and EB1089 on cell growth and on Vitamin D receptor (VDR) mRNA and 1alpha-hydroxylase (1alpha-OHase) mRNA expression in normal canine prostatic primary cultures. Canine prostatic epithelial cells were isolated, cultured, and treated with vehicle (ethanol), calcitriol, 25OHD(3), and EB1089 at 10(-9) and 10(-7)M. The VDR was present in epithelial and stromal cells of the canine prostate gland. 1,25(OH)(2)D(3), 25OHD(3), and EB1089 inhibited epithelial cell growth at 10(-7)M compared to vehicle-treated controls [calcitriol (P < 0.01), EB1089 (P < 0.01), and 25OHD(3) (P < 0.05)]. Epithelial cells treated with calcitriol and EB1089 at 10(-7)M had slightly increased VDR mRNA expression (0.2-0.3-fold) at 6 and 12h compared to controls. There was no difference in 1alpha-OHase mRNA expression in epithelial cells treated with these three compounds. 1,25(OH)(2)D(3) and its analogs may be effective antiproliferative agents of epithelial cells in certain types of prostate cancer.
本研究的目的是调查1,25(OH)₂D₃（骨化三醇）、25OHD₃和EB1089对正常犬前列腺原代培养物中细胞生长以及维生素D受体（VDR）mRNA和1α-羟化酶（1α-OHase）mRNA表达的影响。分离犬前列腺上皮细胞，进行培养，并用赋形剂（乙醇）、骨化三醇、25OHD₃和EB1089在10⁻⁹和10⁻⁷M浓度下进行处理。VDR存在于犬前列腺的上皮细胞和基质细胞中。与赋形剂处理的对照组相比，1,25(OH)₂D₃、25OHD₃和EB1089在10⁻⁷M浓度时抑制上皮细胞生长[骨化三醇（P < 0.01）、EB1089（P < 0.01）和25OHD₃（P < 0.05）]。与对照组相比，在6小时和12小时时，用10⁻⁷M骨化三醇和EB1089处理的上皮细胞VDR mRNA表达略有增加（0.2 - 0.3倍）。用这三种化合物处理的上皮细胞中1α-OHase mRNA表达没有差异。1,25(OH)₂D₃及其类似物可能是某些类型前列腺癌上皮细胞的有效抗增殖剂。
