伤寒沙门氏菌I型3-脱氢奎尼酸酶的结晶

Crystallization of a type I 3-dehydroquinase from Salmonella typhi.

作者信息

Boys C W, Bury S M, Sawyer L, Moore J D, Charles I G, Hawkins A R, Deka R, Kleanthous C, Coggins J R

机构信息

Department of Biochemistry, University of Edinburgh, Scotland.

出版信息

J Mol Biol. 1992 Sep 5;227(1):352-5. doi: 10.1016/0022-2836(92)90704-n.

DOI:10.1016/0022-2836(92)90704-n

PMID:1522599

Abstract

Crystals have been grown of a type I 3-dehydroquinase from both Escherichia coli and Salmonella typhi. However, only those from S. typhi diffract to a resolution of 2.3 A on a conventional X-ray source and are suitable for structure determination. The space group has been determined as P2(1)2(1)2 with unit cell dimensions a = 48.01 A, b = 114.29 A, c = 42.87 A. There is one subunit in the asymmetric unit.

摘要

已经从大肠杆菌和伤寒沙门氏菌中培养出了I型3-脱氢奎尼酸酶晶体。然而，只有来自伤寒沙门氏菌的晶体在传统X射线源上能衍射到2.3埃的分辨率，适合进行结构测定。已确定其空间群为P2(1)2(1)2，晶胞尺寸a = 48.01埃，b = 114.29埃，c = 42.87埃。不对称单元中有一个亚基。