Ing Nancy H, Zhang Yuhua
Departments of Animal Science, Faculty of Genetics, Texas A&M University, College Station, TX 77843-2471, USA.
Theriogenology. 2004 Aug;62(3-4):403-14. doi: 10.1016/j.theriogenology.2003.10.017.
A single physiological dose of estradiol up-regulates estrogen receptor-alpha(ER), progesterone receptor (PR), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), c-fos, cyclophilin, and actin mRNAs in the endometrium of ovariectomized ewes. Therefore, we hypothesized that these genes would be up-regulated by the preovulatory surge of estrogen which occurs on the evening of Day 15 in the estrous cycle of sheep. ER and PR mRNA concentrations increased between Day 15 and Day 1 in cyclic ewes in most endometrial epithelial cells, while GAPDH mRNA increased in epithelial and stromal cells in the deep endometrium. Day 15 pregnant ewes had lower expression of ER, PR, GAPDH, cyclophilin and actin genes. For ER and GAPDH mRNAs, the greatest reduction occurred in the superficial endometrium. Ovariectomized ewes demonstrated concentrations of ER, PR, and GAPDH mRNAs that were similar to those in the cyclic ewes. While concentrations of c-fos mRNA did not differ between groups, those of cyclophilin and actin mRNAs were lower in the pregnant and ovariectomized ewes. In conclusion, ER, PR and GAPDH gene expression rose during estrus in endometrial cells with the highest ER gene expression and were repressed in pregnant ewes in superficial endometrial cells with the greatest PR gene expression.
单一生理剂量的雌二醇可上调去卵巢母羊子宫内膜中的雌激素受体α（ER）、孕激素受体（PR）、甘油醛-3-磷酸脱氢酶（GAPDH）、c-fos、亲环蛋白和肌动蛋白的信使核糖核酸（mRNA）。因此，我们推测这些基因会受到绵羊发情周期第15天晚上出现的排卵前雌激素高峰的上调。在大多数子宫内膜上皮细胞中，ER和PR mRNA浓度在发情周期的母羊第15天到第1天之间升高，而GAPDH mRNA在深层子宫内膜的上皮和基质细胞中增加。第15天的怀孕母羊中，ER、PR、GAPDH、亲环蛋白和肌动蛋白基因的表达较低。对于ER和GAPDH mRNA，最大的降低发生在浅表子宫内膜。去卵巢母羊显示出与发情周期母羊相似的ER、PR和GAPDH mRNA浓度。虽然c-fos mRNA浓度在各组之间没有差异，但亲环蛋白和肌动蛋白mRNA浓度在怀孕和去卵巢母羊中较低。总之，ER、PR和GAPDH基因表达在发情期子宫内膜细胞中升高，其中ER基因表达最高，而在怀孕母羊的浅表子宫内膜细胞中受到抑制，其中PR基因表达最高。
