Lawrence Nathan S, Deo Randhir P, Wang Joseph
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA.
Anal Chem. 2004 Jul 1;76(13):3735-9. doi: 10.1021/ac049943v.
The preparation and advantages of a new generation of carbon paste enzyme electrodes where the redox mediator acts also as the pasting liquid are described. The mediator pasting liquid concept is illustrated for amperometric biosensing of glucose in connection with either the tert-pentylferrocene or n-butylferrocene mediator/binder along with the glucose oxidase enzyme. The attractive performance and advantages of the new device is indicated from comparison to a conventional carbon paste biosensor using a mineral oil binder and the dimethylferrocene electron acceptor. The simplified preparation of the biosensor is coupled with a greatly improved sensitivity and an extended linear range. The mediator pasting liquid imparts high thermal stability onto the embedded enzyme and leads to good resistance to oxygen effects. Owing to the huge mediator reservoir, stability problems associated with the leaching of the mediator are greatly reduced. The fundamental aspects of the electrode behavior have been examined first in the absence of the enzyme. Variables affecting the performance of the new carbon paste biosensor have been investigated and optimized. Such use of the electron acceptor as a binder as well as the mediator offers considerable promise for the biosensing of numerous analytes of clinical and environmental significance.
本文描述了新一代碳糊酶电极的制备方法及其优势，其中氧化还原介质同时充当糊剂液体。以叔戊基二茂铁或正丁基二茂铁介质/粘合剂与葡萄糖氧化酶相结合为例，阐述了介质糊剂液体概念在葡萄糖安培生物传感中的应用。通过与使用矿物油粘合剂和二甲基二茂铁电子受体的传统碳糊生物传感器进行比较，表明了新装置具有吸引人的性能和优势。生物传感器制备过程的简化伴随着灵敏度的大幅提高和线性范围的扩展。介质糊剂液体赋予嵌入酶高的热稳定性，并使其对氧气影响具有良好的抗性。由于存在大量的介质储备，与介质浸出相关的稳定性问题大大减少。首先在没有酶的情况下研究了电极行为的基本方面。对影响新型碳糊生物传感器性能的变量进行了研究和优化。将电子受体既用作粘合剂又用作介质，为临床和环境意义重大的众多分析物的生物传感提供了广阔前景。
