台湾地区采用巢式聚合酶链反应检测扁桃体及非肿瘤性鼻咽部中LMP-1基因的患病率

Prevalence of LMP-1 gene in tonsils and non-neoplastic nasopharynxes by nest-polymerase chain reaction in Taiwan.

作者信息

Pai Ping-Ching, Tsang Ngan-Ming, Tseng Chen-Kan, Hao Sheng-Po, Kuo Tseng-Tong, Wei Kuo-Chen, Hsueh Chuen, Chuang Chi-Cheng

机构信息

Department of Radiation Oncology, Chang Gung Memorial Hospital, 5 Fu-Shin Street, Kwei-Shan Hsiang, Taoyuan, Linkou, Taiwan ROC.

出版信息

Head Neck. 2004 Jul;26(7):619-24. doi: 10.1002/hed.20057.

DOI:10.1002/hed.20057

PMID:15229905

Abstract

BACKGROUND

The purpose of this study was to investigate the frequency of Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1) in tonsils and non-neoplastic nasopharynxes in Taiwan.

METHODS

Nest-polymerase chain reaction (nest-PCR) was used to examine the presence of LMP-1 gene in lymphoid hyperplasia from non-neoplastic tonsillar and nasopharyngeal tissues and in tonsillar cancers.

RESULTS

In 152 cases, 64 biopsy tissues were obtained from lymphoid hyperplasia of nasopharynxes, 57 from tonsillectomy of non-neoplastic tonsils, and 31 from tonsillar cancers. LMP-1 was detected in 43.4% of the study group. Nineteen (29.7%) and 29 (50.9%) lymphoid hyperplasias from normal nasopharynxes and tonsils, respectively, and 18 (58.1%) biopsies from tonsillar cancers had positive LMP-1. The 30-base pair (bp) deleted variant was detected in 89.5% and 82.8% of normal nasopharynxes and tonsils, respectively, and in 66.7% of biopsies from tonsillar cancers (p =.198).

CONCLUSION

This study found that the 30-bp variant was the predominant type of LMP-1 from a healthy population in Taiwan.

摘要

背景

本研究旨在调查台湾地区扁桃体及非肿瘤性鼻咽组织中EB病毒（EBV）潜伏膜蛋白1（LMP-1）的出现频率。

方法

采用巢式聚合酶链反应（nest-PCR）检测非肿瘤性扁桃体和鼻咽组织的淋巴样增生以及扁桃体癌中LMP-1基因的存在情况。

结果

在152例病例中，64份活检组织取自鼻咽部淋巴样增生，57份取自非肿瘤性扁桃体切除组织，31份取自扁桃体癌组织。研究组中LMP-1的检出率为43.4%。正常鼻咽部和扁桃体的淋巴样增生分别有19例（29.7%）和29例（50.9%），扁桃体癌活检组织中有18例（58.1%）LMP-1呈阳性。在正常鼻咽部和扁桃体中分别有89.5%和82.8%检测到30个碱基对（bp）缺失变异体，扁桃体癌活检组织中有66.7%检测到该变异体（p = 0.198）。