Mansoor A, Stevenson M S, Li R Z, Frekko K, Weiss W, Ahmad M, Khan A H, Mushtaq S, Saleem M, Raffeld M, Kingma D W, Jaffe E S
Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1500, USA.
Hum Pathol. 1997 Mar;28(3):283-8. doi: 10.1016/s0046-8177(97)90125-8.
To investigate the potential relationship of socioeconomic status with the prevalence of Epstein-Barr virus (EBV) and to understand the significance of del-LMP-1 within EBV+ cases of Burkitt's lymphoma (BL), we studied 10 cases of BL, 30 cases of diffuse large cell lymphoma (DLCL) arising in nonimmunocompromised patients, and 30 reactive tonsillar biopsy specimens from Pakistan. Each lymphoma was analyzed for EBV by EBER1 RNA in situ hybridization (EBV-RISH). Cases showing hybridization signal within neoplastic cells and all reactive tonsillar tissues were analyzed for EBV strain type by EBNA-2 polymerase chain reaction (PCR) and for the presence of a del-LMP-1 by PCR. Eight of 10 (80%) of BL were EBV+, each containing EBV strain A and a wild-type LMP-1 gene. In contrast, only 4 of 30 DLCL (13%) cases were EBV positive (three strain A, one strain B), each containing a wild-type LMP-1 gene. Fifteen of 30 tonsillar biopsy specimens contained EBV, all of which were strain A and wild-type for LMP1. The prevalence of EBV in BL from Pakistan is slightly lower than in BL in endemic regions, but significantly higher than in BL in North America. EBV positivity probably reflects the socioeconomic status of the patient population and age at seroconversion. The absence of del-LMP-1 within all EBV+ BL cases is consistent with the view that del-LMP-1 is not involved in the pathogenesis of BL, and the presence of del-LMP-1 in EBV+ cases of BL reported in other studies may likely reflect the prevalence of a viral strain containing the 30-bp deletion within the respective population studied.
为了研究社会经济地位与爱泼斯坦-巴尔病毒（EBV）流行率之间的潜在关系，并了解del-LMP-1在EBV阳性的伯基特淋巴瘤（BL）病例中的意义，我们研究了10例BL、30例非免疫功能低下患者发生的弥漫性大细胞淋巴瘤（DLCL）以及来自巴基斯坦的30份反应性扁桃体活检标本。通过EBER1 RNA原位杂交（EBV-RISH）对每个淋巴瘤进行EBV分析。对肿瘤细胞内显示杂交信号的病例以及所有反应性扁桃体组织，通过EBNA-2聚合酶链反应（PCR）分析EBV毒株类型，并通过PCR分析del-LMP-1的存在情况。10例BL中有8例（80%）为EBV阳性，每例均含有EBV毒株A和野生型LMP-1基因。相比之下，30例DLCL中只有4例（13%）为EBV阳性（3例毒株A，1例毒株B），每例均含有野生型LMP-1基因。30份扁桃体活检标本中有15份含有EBV，所有这些标本均为毒株A且LMP1为野生型。巴基斯坦BL中EBV的流行率略低于流行地区的BL，但显著高于北美的BL。EBV阳性可能反映了患者群体的社会经济地位和血清转化时的年龄。所有EBV阳性的BL病例中均不存在del-LMP-1，这与del-LMP-1不参与BL发病机制的观点一致，其他研究报道的EBV阳性BL病例中存在del-LMP-1可能反映了在所研究的各自人群中含有30bp缺失的病毒株的流行情况。
