2'-功能化核酸作为分子生物学中的结构工具。

2'-Functionalized nucleic acids as structural tools in molecular biology.

作者信息

Zatsepin Timofei S, Gait Michael J, Oretskaya Tatiana S

机构信息

Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie gory, Moscow 119992, Russia.

出版信息

IUBMB Life. 2004 Apr;56(4):209-14. doi: 10.1080/15216540410001710570.

DOI:10.1080/15216540410001710570

PMID:15230348

Abstract

Modified oligonucleotides bearing 2'-reactive groups or 2'-conjugated molecules have found wide application as structural tools in molecular biology. Of principal interest has been the use of 2'-reactive oligonucleotides for cross-linking with biomolecules and of 2'-conjugated oligonucleotides in hybridization assays. In this review we compare a range of electrophilic, nucleophilic and photoreactive groups for cross-linking and conjugation studies.

摘要

带有2'-反应基团或2'-共轭分子的修饰寡核苷酸已在分子生物学中作为结构工具得到广泛应用。人们主要感兴趣的是使用2'-反应性寡核苷酸与生物分子进行交联，以及在杂交分析中使用2'-共轭寡核苷酸。在本综述中，我们比较了一系列用于交联和共轭研究的亲电、亲核和光反应基团。