Glünder G, van der Ven H, Foulman A
Clinic for Poultry, School of Veterinary Medicine Hannover, Bunteweg 17, 30559 Hannover, Germany.
Pol J Vet Sci. 2004;7(2):77-81.
For evaluating the influence of the age of the vaccinated birds on the development of antibodies, five groups of turkey poults were inoculated subcutaneously at day 1, 7, 10, 14 and 21 of life with vaccine containing inactivated Bordetella avium and Freund's incomplete adjuvant. No matter which vaccine schedule was used, serum antibodies with the ELISA were first detected at the 28th day of life and increased continuously until the 49th day, when it exhibited either a peak or a plateau. Aluminium hydroxide, Freund's complete and incomplete adjuvant and a mineral oil-arlacel-tween-mixture being permitted adjuvants (appendix II EWG 2377/90) and the adjuvant Gerbu 100 were evaluated for their suitability. Turkeys were vaccinated at the age of three weeks and examined clinically as well as serologically up to the 11th week. Humoral antibodies were detected quantitatively using an ELISA for IgG and a microagglutination test for IgM and qualitatively using immunodiffusion. The damage at the application site was rated by measurement of the swelling of the tissue. In the 10th week, the animals were infected with the agent for challenge. The serological examination for IgG antibodies in the ELISA both treatments with Freund's adjuvants resulted in high titers, which differed significantly from the unvaccinated control after 21 days. IgM could be detected from day 7 onwards in all vaccinated groups and showed the highest titers when aluminium hydroxide was used as adjuvant. In the immunodiffusion assay, precipitating antibodies could be found from the first week after vaccination onwards. There was no correlation between the occurrence of precipitating antibodies and ELISA titers. The measurements of the swelling of the tissue in the beginning showed the largest swellings in the animals injected with Freund's incomplete adjuvant and differed significantly from the unvaccinated control. In the 10th week, the animals were infected with Bordetella avium for challenge. In comparison to the unvaccinated animals, all vaccinated turkeys, no matter which adjuvant was used, showed a distinct and significant reduction of the reisolation rate.
为评估接种疫苗的雏禽年龄对抗体产生的影响，将五组火鸡雏在出生第1天、7天、10天、14天和21天皮下接种含有灭活禽博德特氏菌和弗氏不完全佐剂的疫苗。无论采用哪种疫苗接种方案，用酶联免疫吸附测定法（ELISA）首次检测到血清抗体是在出生第28天，并且抗体水平持续上升直至第49天，此时抗体水平达到峰值或平台期。对氢氧化铝、弗氏完全佐剂和不完全佐剂以及一种矿物油 - 阿拉塞尔 - 吐温混合物（附录II EWG 2377/90中允许使用的佐剂）和Gerbu 100佐剂的适用性进行了评估。三周龄的火鸡接种疫苗，并在第11周前进行临床和血清学检查。使用针对IgG的ELISA法定量检测体液抗体，使用针对IgM的微量凝集试验检测IgM，并使用免疫扩散法定性检测。通过测量组织肿胀程度对注射部位的损伤进行评级。在第10周，让动物感染用于攻毒的病原体。ELISA法检测IgG抗体的血清学检查结果显示，两种使用弗氏佐剂的处理均产生了高滴度抗体，在21天后与未接种疫苗的对照组有显著差异。在所有接种疫苗的组中，从第7天起就可以检测到IgM，当使用氢氧化铝作为佐剂时，IgM滴度最高。在免疫扩散试验中，从接种疫苗后的第一周起就可以发现沉淀抗体。沉淀抗体的出现与ELISA滴度之间没有相关性。最初测量组织肿胀程度时，注射弗氏不完全佐剂的动物肿胀最大，与未接种疫苗的对照组有显著差异。在第10周，让动物感染禽博德特氏菌进行攻毒。与未接种疫苗的动物相比，所有接种疫苗的火鸡，无论使用哪种佐剂，其再分离率均有明显且显著的降低。
