使用集成式扫描电化学显微镜-原子力显微镜（SECM-AFM）探针在原子力显微镜成像过程中通过检测酶促产物来绘制酶活性图谱。
Mapping of enzyme activity by detection of enzymatic products during AFM imaging with integrated SECM-AFM probes.
作者信息
Kranz C, Kueng A, Lugstein A, Bertagnolli E, Mizaikoff B
机构信息
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
出版信息
Ultramicroscopy. 2004 Aug;100(3-4):127-34. doi: 10.1016/j.ultramic.2003.10.004.
With the integration of submicro- and nanoelectrodes into atomic force microscopy (AFM) probes using microfabrication techniques, an elegant approach combining scanning electrochemical microscopy (SECM) with AFM has recently been introduced. Simultaneous contact mode imaging of a micropatterned sample with immobilized enzyme spots and imaging of enzyme activity is shown. In contrast to force spectroscopy the conversion of an enzymatic byproduct is directly detected during AFM imaging and correlated to the activity of the enzyme.
通过使用微加工技术将亚微电极和纳米电极集成到原子力显微镜（AFM）探针中，最近引入了一种将扫描电化学显微镜（SECM）与AFM相结合的精妙方法。展示了对具有固定酶斑的微图案化样品进行同时接触模式成像以及酶活性成像。与力谱法不同，在AFM成像过程中可直接检测酶副产物的转化，并将其与酶的活性相关联。
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